Mouse Succinate Dehydrogenase Complex Subunit C (SDHC) ELISA Kit | SDHC elisa kit

Mouse Succinate Dehydrogenase Complex Subunit C (SDHC) ELISA Kit

Cat. Num. AAA2706834

• Gene Names: SDHC; CYBL; PGL3; QPS1; SDH3; CYB560
• Reactivity: Mouse
• Synonyms: Succinate Dehydrogenase Complex Subunit C (SDHC); Mouse Succinate Dehydrogenase Complex Subunit C (SDHC) ELISA Kit; PGL3; CYB560; SDH3; CYBL; QPs1; Integral membrane protein CII-3; Succinate-ubiquinone oxidoreductase cytochrome B large subunit; Succinate dehydrogenase cytochrome b560; SDHC elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Succinate Dehydrogenase Complex Subunit C (SDHC).; No significant cross-reactivity or interference between Succinate Dehydrogenase Complex Subunit C (SDHC) and analogues was observed.
• Sequence Length: 116

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.071ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Succinate Dehydrogenase Complex Subunit C (SDHC) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Succinate Dehydrogenase Complex Subunit C (SDHC) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SDHC elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Succinate Dehydrogenase Complex Subunit C (SDHC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Succinate Dehydrogenase Complex Subunit C (SDHC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Succinate Dehydrogenase Complex Subunit C (SDHC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Succinate Dehydrogenase Complex Subunit C (SDHC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 78096641
• NCBI GeneID: 6391
• UniProt Accession #: Q99643; O75609; Q3C259; Q3C2D8; Q3C2H4; Q5VTH3
• Molecular Weight: 12,474 Da
• NCBI Official Full Name: succinate dehydrogenase complex, subunit C delta5 alternative splicing variant
• NCBI Official Synonym Full Names: succinate dehydrogenase complex subunit C
• NCBI Official Symbol: SDHC
• NCBI Official Synonym Symbols: CYBL; PGL3; QPS1; SDH3; CYB560
• NCBI Protein Information: succinate dehydrogenase cytochrome b560 subunit, mitochondrial
• UniProt Protein Name: Succinate dehydrogenase cytochrome b560 subunit, mitochondrial
• Protein Family: Succinate dehydrogenase 2 membrane
• UniProt Gene Name: SDHC
• UniProt Synonym Gene Names: CYB560; SDH3; QPs1; CYBL

NCBI Description

This gene encodes one of four nuclear-encoded subunits that comprise succinate dehydrogenase, also known as mitochondrial complex II, a key enzyme complex of the tricarboxylic acid cycle and aerobic respiratory chains of mitochondria. The encoded protein is one of two integral membrane proteins that anchor other subunits of the complex, which form the catalytic core, to the inner mitochondrial membrane. There are several related pseudogenes for this gene on different chromosomes. Mutations in this gene have been associated with paragangliomas. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2013]

Uniprot Description

Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).