Rat Cyclin-dependent kinase 5 ELISA Kit | Cdk5 elisa kit

Rat Cyclin-dependent kinase 5 ELISA Kit

Cat. Num. AAA288634

• Reactivity: Rat
• Synonyms: Cyclin-dependent kinase 5; Rat Cyclin-dependent kinase 5 ELISA Kit; Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit; Cdk5; Cdkn5; 2.7.11.22; Cdk5 elisa kit

• Reactivity: Rat
• Sequence Length: 292

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Cdk5 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18266682
• NCBI GeneID: 140908
• NCBI Accession #: NP_543161.1
• NCBI GenBank Nucleotide #: NM_080885.1
• UniProt Accession #: Q03114
• Molecular Weight: 33,254 Da
• NCBI Official Full Name: cyclin-dependent-like kinase 5
• NCBI Official Synonym Full Names: cyclin-dependent kinase 5
• NCBI Official Symbol: Cdk5
• NCBI Protein Information: cyclin-dependent-like kinase 5
• UniProt Protein Name: Cyclin-dependent-like kinase 5
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: Cdk5
• UniProt Synonym Gene Names: Cdkn5; TPKII catalytic subunit
• UniProt Entry Name: CDK5_RAT

NCBI Description

serine/threonine kinase involved in synaptic regulation and neuronal development; phosphorylates synaptic protein Pctaire1; regulates acetylcholine receptor expression [RGD, Feb 2006]

Uniprot Description

CDK5: a protein kinase of the CDK family. Unlike other members of this family, it is not activated by cyclins but by p35 (CDK5R1) and p39. An important regulator of neuronal positioning during brain development. May also play a role in synaptogenesis and neurotransmission. Substrates include TAU, MAP2, NF-H and -M, Nudel, PDE6, beta-catenin, amphyphysin, dynamin I, synapsin 1, Munc-18, and NMDA receptor 2A. Plays a role in myogenesis, haematopoietic cell differentiation, spermatogenesis, insulin secretion, and lens differentiation. Implicated in the pathology of neurofibrillary tangles and formation of senile plaques, hallmarks of Alzheimer?s disease. Induces tau phosphorylation and aggregation and neurofibrillary tangle deposition and neurodegeneration in in vitro and in vivo animal models. Brain samples from Alzeimer?s pateints show elevated CDK5 activity.

Protein type: Kinase, protein; Protein kinase, CMGC; Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CMGC group; CDK family; CDK5 subfamily; CDK/CDK5 subfamily

Cellular Component: axon; cell junction; cell soma; cyclin-dependent protein kinase 5 activator complex; cytoplasm; cytoskeleton; cytosol; dendrite; filopodium; growth cone; lamellipodium; membrane; neuromuscular junction; nucleolus; nucleus; perikaryon; plasma membrane; postsynaptic density; postsynaptic membrane

Molecular Function: acetylcholine receptor activator activity; ATP binding; cyclin-dependent protein kinase activity; cytoskeletal protein binding; ephrin receptor binding; ErbB-2 class receptor binding; ErbB-3 class receptor binding; kinase activity; p53 binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau-protein kinase activity

Biological Process: apoptosis; associative learning; axon extension; axonogenesis; behavioral response to cocaine; cell division; cell migration; cell-matrix adhesion; central nervous system development; central nervous system neuron development; cerebellar cortex development; cerebellar cortex formation; cerebellum development; cerebral cortex development; corpus callosum development; cortical actin cytoskeleton organization and biogenesis; dendrite morphogenesis; exocytosis; forebrain development; hippocampus development; intracellular protein transport; layer formation in the cerebral cortex; motor axon guidance; negative regulation of axon extension; negative regulation of cell cycle; negative regulation of protein export from nucleus; negative regulation of protein ubiquitination; negative regulation of proteolysis; negative regulation of synaptic plasticity; negative regulation of transcription, DNA-dependent; neurite development; neurite morphogenesis; neuron apoptosis; neuron differentiation; neuron migration; nucleocytoplasmic transport; oligodendrocyte differentiation; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of calcium ion-dependent exocytosis; positive regulation of neuron apoptosis; positive regulation of protein binding; positive regulation of protein kinase activity; protein amino acid autophosphorylation; protein amino acid phosphorylation; receptor catabolic process; receptor clustering; regulated secretory pathway; regulation of cell migration; regulation of excitatory postsynaptic membrane potential; regulation of postsynaptic membrane potential; regulation of synaptic plasticity; response to cocaine; response to wounding; rhythmic process; Schwann cell development; sensory perception of pain; serine phosphorylation of STAT3 protein; skeletal muscle development; synaptic transmission, dopaminergic; synaptic transmission, glutamatergic; synaptic vesicle endocytosis; synaptogenesis; telencephalon development; visual learning