Human Cyclin-dependent kinase 5 ELISA Kit | CDK5 elisa kit

Human Cyclin-dependent kinase 5 ELISA Kit

Cat. Num. AAA760218

• Gene Names: CDK5; LIS7; PSSALRE
• Reactivity: Human
• Synonyms: Cyclin-dependent kinase 5; Human Cyclin-dependent kinase 5 ELISA Kit; CDK5 (Cyclin-dependent kinase 5)/TPKII catalytic subunit/Tau protein kinase II catalytic subunit/Cell division protein kinase 5/Serine/threonine-protein kinase PSSALRE; CDK5 elisa kit

• Reactivity: Human
• Sequence Length: 292

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CDK5 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human CDK5antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human CDK5 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CDK5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman CDK5can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 16356641
• NCBI GeneID: 1020
• Molecular Weight: 29,544 Da
• NCBI Official Full Name: cyclin-dependent kinase 5
• NCBI Official Synonym Full Names: cyclin-dependent kinase 5
• NCBI Official Symbol: CDK5
• NCBI Official Synonym Symbols: LIS7; PSSALRE
• NCBI Protein Information: cyclin-dependent-like kinase 5
• UniProt Protein Name: Cyclin-dependent-like kinase 5
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK5
• UniProt Synonym Gene Names: CDKN5; TPKII catalytic subunit
• UniProt Entry Name: CDK5_HUMAN

NCBI Description

This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]

Uniprot Description

CDK5: a protein kinase of the CDK family. Unlike other members of this family, it is not activated by cyclins but by p35 (CDK5R1) and p39. An important regulator of neuronal positioning during brain development. May also play a role in synaptogenesis and neurotransmission. Substrates include TAU, MAP2, NF-H and -M, Nudel, PDE6, beta-catenin, amphyphysin, dynamin I, synapsin 1, Munc-18, and NMDA receptor 2A. Plays a role in myogenesis, haematopoietic cell differentiation, spermatogenesis, insulin secretion, and lens differentiation. Implicated in the pathology of neurofibrillary tangles and formation of senile plaques, hallmarks of Alzheimer?s disease. Induces tau phosphorylation and aggregation and neurofibrillary tangle deposition and neurodegeneration in in vitro and in vivo animal models. Brain samples from Alzeimer?s pateints show elevated CDK5 activity.

Protein type: Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; EC 2.7.11.1; CMGC group; CDK family; CDK/CDK5 subfamily; CDK5 subfamily

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: axon; cell junction; cell soma; cyclin-dependent protein kinase 5 activator complex; cytoplasm; cytoskeleton; cytosol; dendrite; filopodium; growth cone; lamellipodium; membrane; neuromuscular junction; nucleus; perikaryon; plasma membrane; postsynaptic density; postsynaptic membrane

Molecular Function: acetylcholine receptor activator activity; ATP binding; cyclin-dependent protein kinase activity; cytoskeletal protein binding; ephrin receptor binding; ErbB-2 class receptor binding; ErbB-3 class receptor binding; kinase activity; p53 binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau-protein kinase activity

Biological Process: axon extension; axon guidance; behavioral response to cocaine; blood coagulation; cell cycle; cell division; cell proliferation; cell-matrix adhesion; central nervous system neuron development; cerebellar cortex formation; corpus callosum development; cortical actin cytoskeleton organization and biogenesis; dendrite morphogenesis; hippocampus development; intracellular protein transport; layer formation in the cerebral cortex; motor axon guidance; negative regulation of axon extension; negative regulation of cell cycle; negative regulation of protein export from nucleus; negative regulation of protein ubiquitination; negative regulation of proteolysis; negative regulation of synaptic plasticity; negative regulation of transcription, DNA-dependent; neurite development; neuron apoptosis; neuron differentiation; neuron migration; nucleocytoplasmic transport; oligodendrocyte differentiation; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of calcium ion-dependent exocytosis; positive regulation of neuron apoptosis; positive regulation of protein binding; positive regulation of protein kinase activity; protein amino acid autophosphorylation; receptor catabolic process; receptor clustering; regulation of apoptosis; regulation of cell migration; regulation of excitatory postsynaptic membrane potential; regulation of synaptic plasticity; rhythmic process; Schwann cell development; sensory perception of pain; serine phosphorylation of STAT3 protein; skeletal muscle development; synaptic transmission; synaptic transmission, dopaminergic; synaptic transmission, glutamatergic; synaptic vesicle endocytosis; synaptic vesicle exocytosis; synaptogenesis; visual learning

Disease: Lissencephaly 7 With Cerebellar Hypoplasia