Cyclin Dependent Kinase 1 (CDK1) Recombinant Protein | CDK1 recombinant protein

Recombinant Cyclin Dependent Kinase 1 (CDK1)

Cat. Num. AAA2010607

• Gene Names: CDK1; CDC2; CDC28A; P34CDC2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cyclin Dependent Kinase 1 (CDK1); Recombinant Cyclin Dependent Kinase 1 (CDK1); CDK1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-RH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM DSSLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR YS
• Sequence Length: 297

• Applicable Applications for CDK1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Arg59~Ser182 (Accession # P06493) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4502709
• NCBI GeneID: 983
• NCBI Accession #: NP_001777.1
• NCBI GenBank Nucleotide #: NM_001786.4
• UniProt Accession #: P06493; O60764; A8K7C4; C9J497
• Molecular Weight: 15.9kDa
• NCBI Official Full Name: cyclin-dependent kinase 1 isoform 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 1
• NCBI Official Symbol: CDK1
• NCBI Official Synonym Symbols: CDC2; CDC28A; P34CDC2
• NCBI Protein Information: cyclin-dependent kinase 1; p34 protein kinase; cell cycle controller CDC2; cell division protein kinase 1; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M
• UniProt Protein Name: Cyclin-dependent kinase 1
• UniProt Gene Name: CDK1
• UniProt Synonym Gene Names: CDC2; CDC28A; CDKN1; P34CDC2; CDK1
• UniProt Entry Name: CDK1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Motility/polarity/chemotaxis; Protein kinase, CMGC; EC 2.7.11.22; EC 2.7.11.23; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: nucleoplasm; centrosome; membrane; mitochondrion; spindle microtubule; cytoplasm; midbody; nucleus; cytosol

Molecular Function: RNA polymerase subunit kinase activity; protein serine/threonine kinase activity; cyclin binding; protein binding; cyclin-dependent protein kinase activity; histone kinase activity; Hsp70 protein binding; ATP binding; protein kinase activity

Biological Process: regulation of Schwann cell differentiation; nerve growth factor receptor signaling pathway; activation of MAPKK activity; activation of MAPK activity; histone phosphorylation; response to toxin; regulation of embryonic development; ventricular cardiac muscle cell development; stress-activated MAPK cascade; centrosome cycle; toll-like receptor 3 signaling pathway; response to organic cyclic substance; toll-like receptor 5 signaling pathway; small GTPase mediated signal transduction; protein complex assembly; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; positive regulation of cardiac muscle cell proliferation; response to drug; mitosis; organ regeneration; fibroblast growth factor receptor signaling pathway; positive regulation of protein import into nucleus, translocation; cell aging; pronuclear fusion; toll-like receptor 2 signaling pathway; chromosome condensation; response to ethanol; cell division; toll-like receptor 9 signaling pathway; response to activity; G1/S transition of mitotic cell cycle; response to amine stimulus; negative regulation of apoptosis; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; axon guidance; apoptosis; mitotic nuclear envelope disassembly; positive regulation of mitotic cell cycle; toll-like receptor 10 signaling pathway; epithelial cell differentiation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; response to axon injury; DNA replication; epidermal growth factor receptor signaling pathway; cell migration; organelle organization and biogenesis; MyD88-independent toll-like receptor signaling pathway; peptidyl-threonine phosphorylation; MAPKKK cascade; microtubule cytoskeleton organization and biogenesis; DNA repair; MyD88-dependent toll-like receptor signaling pathway; G1/S-specific transcription in mitotic cell cycle; peptidyl-serine phosphorylation; response to cadmium ion; response to copper ion; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic cell cycle G2/M transition DNA damage checkpoint; Ras protein signal transduction; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; positive regulation of DNA replication