Rabbit anti-Human, Mouse EHD2 Polyclonal Antibody | anti-EHD2 antibody

EHD2 Antibody

Cat. Num. AAA7051256

• Gene Names: EHD2; PAST2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: EHD2; Polyclonal Antibody; EHD2 Antibody; EH domain-containing protein 2Curated; PAST homolog 2Curated; anti-EHD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 543

• Applicable Applications for anti-EHD2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1: 500-1: 5000; IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant Human EH domain-containing protein 2 protein (401-543AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-70263 / sc-70264 / sc-70266
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Hela whole cell lysate,Mouse liver tissue,Mouse kidney tissueAll lanes: EHD2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 62,47 kDaObserved band size: 62,47 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7051256 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS7051256 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-EHD2 antibody

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (By similarity). Plays a role in membrane trafficking between the plasma membrane and endosomes (PubMed: 17233914). Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 21361462
• NCBI GeneID: 30846
• NCBI Accession #: NP_055416.2
• NCBI GenBank Nucleotide #: NM_014601.3
• UniProt Accession #: Q9NZN4; Q96CB6; B2RDH9; B4DNU6
• Molecular Weight: 46,059 Da
• NCBI Official Full Name: EH domain-containing protein 2
• NCBI Official Synonym Full Names: EH domain containing 2
• NCBI Official Symbol: EHD2
• NCBI Official Synonym Symbols: PAST2
• NCBI Protein Information: EH domain-containing protein 2
• UniProt Protein Name: EH domain-containing protein 2
• Protein Family: EH domain-containing protein
• UniProt Gene Name: EHD2
• UniProt Entry Name: EHD2_HUMAN

NCBI Description

This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

EHD2: Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: caveola; cytosol; endosome membrane; extrinsic to membrane; nucleus; plasma membrane; recycling endosome membrane

Molecular Function: nucleic acid binding; protein binding

Biological Process: blood coagulation; endocytic recycling