APC/C activator protein CDC20 (CDC20) Recombinant Protein | CDC20 recombinant protein

Recombinant Saccharomyces cerevisiae APC/C activator protein CDC20 (CDC20) , partial

• Gene Names: CDC20; PAC5
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: APC/C activator protein CDC20 (CDC20); Recombinant Saccharomyces cerevisiae APC/C activator protein CDC20 (CDC20); partial; CDC20 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 610

• Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 6321322
• NCBI GeneID: 852762
• NCBI Accession #: NP_011399.1
• NCBI GenBank Nucleotide #: NM_001180981.1
• UniProt Accession #: P26309; D6VU31
• Molecular Weight: 67,360 Da
• NCBI Official Full Name: ubiquitin-protein transferase activating protein CDC20
• NCBI Official Symbol: CDC20
• NCBI Official Synonym Symbols: PAC5
• NCBI Protein Information: ubiquitin-protein transferase activating protein CDC20
• UniProt Protein Name: APC/C activator protein CDC20
• Protein Family: APC/C activator protein
• UniProt Gene Name: CDC20

Uniprot Description

Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). At the metaphase-to-anaphase transition, recognizes and binds proteins containing a D-box including the B-type cyclins CLB2 and CLB5, HSL1 and securin PDS1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for sister chromatid separation and disassembly of the mitotic spindle. Target of the spindle checkpoint pathway through participation in the mitotic checkpoint complex (MCC) and the MAD2-CDC20 subcomplex. MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20 until proper attachment of all chromosomes to the spindle.