Cluster Of Differentiation 8a (CD8a) Recombinant Protein | CD8a recombinant protein

Recombinant Cluster Of Differentiation 8a (CD8a)

Cat. Num. AAA2009862

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cluster Of Differentiation 8a (CD8a); Recombinant Cluster Of Differentiation 8a (CD8a); CD8a recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-GQLQL SPKKVDAEIG QEVKLTCEVL RDTSQGCSWL FRNSSSELLQ PTFIIYVSSS RSKLNDILDP NLFSARKENN KYILTLSKFS TKNQGYYFCS ITSNSVMYFS PLVPVFQKVN SIITKPVTRA PTPVPPPTGT PRPLRPEACR PGASGSVEGM GLGFACDIYI WAPLAGICAV LLLSLVITLI CCHRNRRRVC KCPRPLVKPR PSEKFV
• Sequence Length: 236

• Applicable Applications for CD8a recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Gly26~Val236 (Accession # P07725) with N-terminal His-Tag
• Subcellular Location: Membrane; Single-pass type I membrane protein
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 9.5
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 13928726
• NCBI GeneID: 24930
• NCBI Accession #: NP_113726.1
• NCBI GenBank Nucleotide #: NM_031538.2
• UniProt Accession #: P07725
• Molecular Weight: 24.9kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain
• NCBI Official Synonym Full Names: CD8a molecule
• NCBI Official Symbol: Cd8a
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain; OX-8 membrane antigen; CD8 antigen alpha-chain; CD8 antigen 32 kDa chain; CD8 antigen, alpha chain; CD8 antigen, alpha-chain
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: Cd8a
• UniProt Entry Name: CD8A_RAT

NCBI Description

increased expression on mast cells is induced by nitric oxide; may play a role in inflammatory response [RGD, Feb 2006]

Uniprot Description

CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: cell surface; integral to membrane; external side of plasma membrane

Molecular Function: protein homodimerization activity; protein kinase binding

Biological Process: cell surface receptor linked signal transduction; T cell activation; T cell mediated immunity; cytotoxic T cell differentiation; positive regulation of calcium-mediated signaling; inflammatory response; defense response to virus