Rat T-cell surface glycoprotein CD8 alpha chain ELISA Kit | Cd8a elisa kit

Rat T-cell surface glycoprotein CD8 alpha chain ELISA Kit

Cat. Num. AAA761148

• Gene Names: CD8A; CD8; MAL; p32; Leu2
• Reactivity: Rat
• Synonyms: T-cell surface glycoprotein CD8 alpha chain; Rat T-cell surface glycoprotein CD8 alpha chain ELISA Kit; Cd8a/T-cell surface glycoprotein CD8 alpha chain/T-lymphocyte differentiation antigen T8/Leu-2; Cd8a elisa kit

• Reactivity: Rat
• Sequence Length: 235

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Cd8a elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Cd8a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Cd8a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Cd8a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofRat Cd8a can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 225007536
• NCBI GeneID: 925
• NCBI Accession #: NP_001139345.1
• NCBI GenBank Nucleotide #: NM_001145873.1
• Molecular Weight: 30,214 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain isoform 1
• NCBI Official Synonym Full Names: CD8a molecule
• NCBI Official Symbol: CD8A
• NCBI Official Synonym Symbols: CD8; MAL; p32; Leu2
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD8A
• UniProt Synonym Gene Names: MAL
• UniProt Entry Name: CD8A_HUMAN

NCBI Description

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: external side of plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: cell surface receptor linked signal transduction; cytotoxic T cell differentiation; regulation of immune response; T cell mediated immunity

Disease: Cd8 Deficiency, Familial