CD80/B7-1 recombinant protein

Human CD80/B7-1 protein, His tag (Animal-Free)

Cat. Num. AAA9719066

• Gene Names: CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD80/B7-1; Human CD80/B7-1 protein; His tag (Animal-Free); CD80; Activation B7-1 antigen; B7; BB1; CD28LG1; CD28LGB7-1 antigen; T-lymphocyte activation antigen CD80; CD80/B7-1 recombinant protein

• Host: HEK293 cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4.
• Sequence Positions: 214aa
• Sequence: Met1-Asn242

• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method.
• Protein Construction: Amino acid sequence derived from human CD80 (P33681-1) (Met1-Asn242) was expressed with a 6 His tag at the C-terminus.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CD80/B7-1 protein using the buffer we provided not less than 100 ug/mL, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized Human CD80/B7-1 protein product should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for CD80/B7-1 recombinant protein

Recombinant Human CD80/B7-1 protein expressed in HEK293 Cells.
Background: B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. Mature mouse B7-1 consists of a 209 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 22 aa transmembrane segment, and a 38 aa cytoplasmic domain. Within the ECD, mouse B7-1 shares 50% and 68% aa sequence identity with human and rat B7-1, respectively. Alternative splicing generates a short isoform that lacks the second Ig-like domain but retains the ability to bind CD28. Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells.

Product Categories/Family for CD80/B7-1 recombinant protein

Cell Culture

NCBI and Uniprot Product Information

• NCBI GI #: 461606
• NCBI GeneID: 941
• UniProt Accession #: P33681; Q5DTA9; Q5DTB0
• Molecular Weight: 18,761 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD80
• NCBI Official Synonym Full Names: CD80 molecule
• NCBI Official Symbol: CD80
• NCBI Official Synonym Symbols: B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• NCBI Protein Information: T-lymphocyte activation antigen CD80
• UniProt Protein Name: T-lymphocyte activation antigen CD80
• UniProt Gene Name: CD80
• UniProt Synonym Gene Names: CD28LG; CD28LG1; LAB7; B7

NCBI Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

Uniprot Description

Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.