CD79 Recombinant Protein | CD79A&CD79B recombinant protein

CD79 Protein, Human, Recombinant (DYKDDDDK & His Tag)

Cat. Num. AAA8123629

• Gene Names: CD79A; IGA; MB-1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CD79; CD79 Protein; Human; Recombinant (DYKDDDDK & His Tag); Human CD79 (CD79A & CD79B Heterodimer) Protein (DYKDDDDK & His Tag); IGA Protein; MB-1 Protein; CD79a molecule; immunoglobulin-associated alpha; CD79A&CD79B recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg143&Met1-Asp159

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Leu 33 & Ala 29
• Tag: C-DYKDDDDK & C-His
• Protein Construction: A DNA sequence encoding the human CD79A (NP_001774.1)(Met1-Arg143) was fused with a DYKDDDDK tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding human CD79B (NP_000617.1)(Met1-Asp159) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD79A & CD79B heterodimer was purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4502685
• NCBI GeneID: 973
• NCBI Accession #: NP_001774.1; NP_000617.1
• NCBI GenBank Nucleotide #: NM_001783.3
• UniProt Accession #: P11912; Q53FB8; A0N775
• Molecular Weight: 25,038 Da
• NCBI Official Full Name: B-cell antigen receptor complex-associated protein alpha chain isoform 1
• NCBI Official Synonym Full Names: CD79a molecule, immunoglobulin-associated alpha
• NCBI Official Symbol: CD79A
• NCBI Official Synonym Symbols: IGA; MB-1
• NCBI Protein Information: B-cell antigen receptor complex-associated protein alpha chain; ig-alpha; MB-1 membrane glycoprotein; surface IgM-associated protein; CD79a antigen (immunoglobulin-associated alpha); membrane-bound immunoglobulin-associated protein
• UniProt Protein Name: B-cell antigen receptor complex-associated protein alpha chain
• UniProt Gene Name: CD79A
• UniProt Synonym Gene Names: IGA; MB1
• UniProt Entry Name: CD79A_HUMAN

NCBI Description

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Ig-alpha: an integral membrane protein that associates with surface immunoglobulin B lymphocyte antigen receptor multimeric complex. Surface Ig non-covalently associates with 2 other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. Two alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: multivesicular body; integral to membrane; plasma membrane; B cell receptor complex; lipid raft; external side of plasma membrane

Molecular Function: transmembrane receptor activity

Biological Process: B cell proliferation; B cell receptor signaling pathway; B cell activation; B cell differentiation

Disease: Agammaglobulinemia 3, Autosomal Recessive