Human HLA class II histocompatibility antigen gamma chain ELISA Kit | CD74 elisa kit

Human HLA class II histocompatibility antigen gamma chain ELISA Kit

Cat. Num. AAA764425

• Gene Names: CD74; II; DHLAG; HLADG; Ia-GAMMA
• Reactivity: Human
• Synonyms: HLA class II histocompatibility antigen gamma chain; Human HLA class II histocompatibility antigen gamma chain ELISA Kit; CD74 (HLA class II histocompatibility antigen gamma chain)/CLIP/DHLAG/HLADG/Ii/II/MHC HLA-DR gamma chain/p33/Ia-gamma/INVG34/CD74 antigen/HLA-DR antigens-associated invariant chain/HLA-DR-gamma/Ia antigen-associated invariant chain/Ia-associated invariant chain; CD74 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CD74. No significant cross-reactivity or interference between CD74 and analogues was observed.
• Sequence Length: 296

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD74 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 68448544
• NCBI GeneID: 972
• NCBI Accession #: NP_001020330.1
• NCBI GenBank Nucleotide #: NM_001025159.2
• Molecular Weight: 18,328 Da
• NCBI Official Full Name: HLA class II histocompatibility antigen gamma chain isoform a
• NCBI Official Synonym Full Names: CD74 molecule
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain
• UniProt Protein Name: HLA class II histocompatibility antigen gamma chain
• Protein Family: H-2 class II histocompatibility antigen
• UniProt Gene Name: CD74
• UniProt Synonym Gene Names: DHLAG; Ii
• UniProt Entry Name: HG2A_HUMAN

NCBI Description

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

CD74: Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. A chromosomal aberration involving CD74 is found in a non-small cell lung tumor. Results in the formation of a CD74- ROS1 chimeric protein. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: cell surface; Golgi membrane; integral to membrane; intracellular; lysosomal lumen; lysosomal membrane; membrane; MHC class II protein complex; plasma membrane; trans-Golgi network membrane; vacuole

Molecular Function: beta-amyloid binding; cytokine binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; MHC class II protein binding; MHC class II protein binding, via antigen binding groove; protein binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; cell proliferation; immunoglobulin mediated immune response; intracellular protein transport; leukocyte migration; negative regulation of apoptosis; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of peptide secretion; positive regulation of B cell proliferation; positive regulation of cytokine and chemokine mediated signaling pathway; positive regulation of fibroblast proliferation; positive regulation of peptidyl-tyrosine phosphorylation; prostaglandin biosynthetic process; protein complex assembly; signal transduction