CD6 recombinant protein

CD6 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8118861

• Purity: >85 % as determined by SDS-PAGE
• Synonyms: CD6; CD6 Protein; Mouse; Recombinant (hFc Tag); Mouse CD6/TP120 Protein (Fc Tag); CD6 molecule; CD6 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 621aa
• Sequence: Met1-Val243

• Species: Mouse
• Tag: hFc
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly17
• Protein Construction: |A DNA sequence encoding the extracellular domain of mouse CD6 (Q91WN5) (Met 1-Val 243) was fused with the Fc region of human IgG1 at the C-terminus
• Activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8 x 104 cells/well are added to mCD6-Fc coated plates (10ug/mL, 100uL/well), approximately 14.4% will adhere after 60 minutes at 37 degree C.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Related Product Information for CD6 recombinant protein

T-cell differentiation antigen CD6, also known as TP12 and CD6, is a single-pass type I membrane protein which contains threeSRCR domains. CD6/TP12 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6/TP12 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6/TP12 binds to activated leukocyte cell adhesion molecule (CD166), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6/TP12 partially associates with the TCR/CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.

Product Categories/Family for CD6 recombinant protein

ITIM/ITAM Immunoreceptors and Related Molecules

References

Joo YS. et al., 2000, Arthritis Rheum. 43 (2): 329-35. Singer NG. et al., 2002, Int Immunol. 14 (6): 585-97. Gimferrer I. et al., 2005, J Immunol. 175 (3): 1406-14. Alonso R. et al., 2010, J Autoimmun. 35 (4): 336-41.

NCBI and Uniprot Product Information

• NCBI GI #: 81879611
• UniProt Accession #: Q91WN5
• Molecular Weight: 72,284 Da
• NCBI Official Full Name: CD6 antigen
• UniProt Protein Name: CD6 antigen
• Protein Family: CD63 antigen
• UniProt Gene Name: Cd6