CD40 recombinant protein

CD40-muIg

Cat. Num. AAA666918

• Gene Names: CD40; p50; Bp50; CDW40; TNFRSF5
• Applications: Flow Cytometry, Functional Assay, ELISA
• Purity: Protein A purified recombinant protein from tissue culture supernatant was conjugated to Allophycocyanin through a sulfo-ester linkage. Unconjugated protein was removed using size exclusion chromatography. Protein conjugate is at a concentration of 0.1 mg
• Synonyms: CD40; CD40-muIg; CD40-muIgAllophycocyanin; Human CD40 muIg/APC Fusion Protein*; CD40 recombinant protein

• Purity/Purification: Protein A purified recombinant protein from tissue culture supernatant was conjugated to Allophycocyanin through a sulfo-ester linkage. Unconjugated protein was removed using size exclusion chromatography. Protein conjugate is at a concentration of 0.1 mg
• Form/Format: Allophycocyanin
• Sequence Length: 117

• Applicable Applications for CD40 recombinant protein: Flow Cytometry (FC/FACS), ELISA (EIA)
• Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Performance: Human Jurkat-4G cells were stimulated 6 hours in culture in the presence of 10 ng/ml PMA and 1uM Ionomicin to up regulate CD154 expression. Five x 105 cells per tube were washed and incubated 45 minutes on ice with 80 ul of a 1:20 dilution (5 ug/ml) of CD40-muIg/APC. They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.33 log10 fluorescent units when compared to a Mouse IgG2a/APC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD154 antibody.
• Preparation and Storage: Store at 2 - 5 degree C. Do not freeze! Protect from light.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Flow Cytometry (FC/FACS)

Related Product Information for CD40 recombinant protein

Human CD40 is a member of the tumor necrosis factor (TNF) receptor family and is present on all B cells except plasma cells. CD40 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 plays an important role in B cell activation and the interaction with its ligand CD154 (CD40 Ligand) is essential for isotype switching (1).

Product Categories/Family for CD40 recombinant protein

Recombinant Proteins

References

1. T.A. Foy, et al, (1996) Annu Rev Immunol 14: 591-617. 2. M.E. Ozaki, et al, (1997) J Immunol 159: 214-221

NCBI and Uniprot Product Information

• NCBI GI #: 114053977
• NCBI GeneID: 958
• Molecular Weight: 22,259 Da
• NCBI Official Full Name: CD40, partial
• NCBI Official Synonym Full Names: CD40 molecule
• NCBI Official Symbol: CD40
• NCBI Official Synonym Symbols: p50; Bp50; CDW40; TNFRSF5
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 5
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 5
• Protein Family: CD40 ligand
• UniProt Gene Name: CD40
• UniProt Synonym Gene Names: TNFRSF5
• UniProt Entry Name: TNR5_HUMAN

NCBI Description

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

Uniprot Description

CD40: a member of the TNF-receptor superfamily. This receptor for CD40L mediates a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3). HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. Two alternatively spliced isoforms have been reported. Isoform I is a type I membrane protein; isoform II is secreted.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 20q12-q13.2

Cellular Component: cell surface; cytoplasm; external side of plasma membrane; extracellular space; integral to plasma membrane; intracellular membrane-bound organelle; plasma membrane

Molecular Function: antigen binding; enzyme binding; protein binding; receptor activity; signal transducer activity; tumor necrosis factor receptor activity; ubiquitin protein ligase binding

Biological Process: activation of NF-kappaB transcription factor; B cell proliferation; cellular calcium ion homeostasis; defense response to protozoan; defense response to virus; immune response; immune response-regulating cell surface receptor signaling pathway; inflammatory response; multicellular organismal development; platelet activation; positive regulation of B cell proliferation; positive regulation of GTPase activity; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; positive regulation of isotype switching to IgG isotypes; positive regulation of MAP kinase activity; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat1 protein; protein complex assembly; protein kinase B signaling cascade; regulation of cell proliferation; regulation of immune response; regulation of immunoglobulin secretion; response to lipopolysaccharide; tumor necrosis factor-mediated signaling pathway

Disease: Immunodeficiency With Hyper-igm, Type 3