Cluster Of Differentiation 4 (CD4) Recombinant Protein | CD4 recombinant protein

Recombinant Cluster Of Differentiation 4 (CD4)

Cat. Num. AAA2009885

• Gene Names: Cd4; L3T4; Ly-4
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cluster Of Differentiation 4 (CD4); Recombinant Cluster Of Differentiation 4 (CD4); CD4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-KTLV LGKEGESAEL PCESSQKKIT VFTWKFSDQR KILGQHGKGV LIRGGSPSQF DRFDSKKGAW EKGSFPLIIN KLKMEDSQTY ICELENRKEE VELWVFKVTF SPGTSLLQGQ SLTLTLDSNS KVSNPLTECK HKKGKVVSGS KVLSMSNLRV QDSDFWNCTV TLDQKKNWFG MTLSVLGFQS TAITAYKSEG ESAEFSFPLN FAEENGWGEL MWKAEKDSFF QPWISFSIKN KEVSVQKSTK DLKLQLKETL PLTLKIPQVS LQFAGSGNLT LTLDKGTLHQ EVNLVVMKVA QLNNTLTCEV MGPTSPKMRL TLKQENQEAR VSEEQKVVQV VAPETGLWQC LLSEGDKVKM DSRIQVLSRG VNQT
• Sequence Length: 457

• Applicable Applications for CD4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Lys27~Thr394 (Accession # P06332) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 7304953
• NCBI GeneID: 12504
• NCBI Accession #: NP_038516.1
• NCBI GenBank Nucleotide #: NM_013488.2
• UniProt Accession #: P06332
• Molecular Weight: 47.0kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD4
• NCBI Official Synonym Full Names: CD4 antigen
• NCBI Official Symbol: Cd4
• NCBI Official Synonym Symbols: L3T4; Ly-4
• NCBI Protein Information: T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; T-cell differentiation antigen L3T4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: Cd4
• UniProt Entry Name: CD4_MOUSE

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Cell surface; Membrane protein, integral

Cellular Component: endoplasmic reticulum membrane; cell surface; membrane; endoplasmic reticulum lumen; integral to membrane; plasma membrane; lipid raft; external side of plasma membrane

Molecular Function: protein binding; enzyme binding; protein homodimerization activity; zinc ion binding; coreceptor activity; glycoprotein binding; protein kinase binding

Biological Process: maintenance of cellular protein localization; T cell activation; immune system process; T cell selection; cytokine production; positive regulation of calcium-mediated signaling; defense response to Gram-negative bacterium; induction by virus of cell-cell fusion in host; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; protein palmitoleylation; immune response; positive regulation of T cell activation; cell adhesion; regulation of T cell activation; T cell differentiation