Sheep Cluster of differentiation 34 ELISA Kit | CD34 elisa kit

Sheep Cluster of differentiation 34 ELISA Kit

Cat. Num. AAA743711

• Reactivity: Sheep
• Synonyms: Cluster of differentiation 34; Sheep Cluster of differentiation 34 ELISA Kit; CD34 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This CD34 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep CD34. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD34 elisa kit

Principle of the Assay: CD34 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD34 antibody and an CD34-HRP conjugate. The assay sample and buffer are incubated together with CD34-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD34 concentration since CD34 from samples and CD34-HRP conjugate compete for the anti-CD34 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD34 from the sample, fewer sites are left to bind CD34-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD34 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CD34 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 264769
• NCBI GeneID: 947
• Molecular Weight: 35,011 Da
• NCBI Official Full Name: CD34
• NCBI Official Synonym Full Names: CD34 molecule
• NCBI Official Symbol: CD34
• NCBI Protein Information: hematopoietic progenitor cell antigen CD34; CD34 antigen
• UniProt Protein Name: Hematopoietic progenitor cell antigen CD34
• Protein Family: Hematopoietic progenitor cell antigen
• UniProt Gene Name: CD34
• UniProt Entry Name: CD34_HUMAN

NCBI Description

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CD34: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Belongs to the CD34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: intercellular bridge; perinuclear region of cytoplasm; integral to plasma membrane; lysosome; apical plasma membrane; cytoplasm; plasma membrane; basal plasma membrane; external side of plasma membrane

Molecular Function: sulfate binding; carbohydrate binding; transcription factor binding

Biological Process: positive regulation of odontogenesis; regulation of immune response; negative regulation of nitric oxide biosynthetic process; glomerular filtration; cell motility involved in cell locomotion; negative regulation of blood coagulation; negative regulation of tumor necrosis factor production; signal transduction; positive regulation of interleukin-10 production; cell proliferation; positive regulation of angiogenesis; cell-cell adhesion; regulation of blood pressure; endothelial cell proliferation; tissue homeostasis; hemopoiesis; leukocyte migration