Sialic Acid Binding Ig-Like Lectin 3/Siglec-3/CD33 Recombinant Protein | Siglec-3/CD33 recombinant protein

Recombinant Human Sialic Acid Binding Ig-Like Lectin 3/Siglec-3/CD33 Protein (C-Fc-6His)

Cat. Num. AAA2553232

• Gene Names: CD33; p67; SIGLEC3; SIGLEC-3
• Purity: >90% as determined by reducing SDS-PAGE.
• Synonyms: Sialic Acid Binding Ig-Like Lectin 3/Siglec-3/CD33; Recombinant Human Sialic Acid Binding Ig-Like Lectin 3/Siglec-3/CD33 Protein (C-Fc-6His); Myeloid Cell Surface Antigen CD33; Sialic Acid-Binding Ig-Like Lectin 3; Siglec-3; gp67; CD33; SIGLEC3; Siglec-3/CD33 recombinant protein

• Host: Human Cells
• Purity/Purification: >90% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, 2mM EDTA, pH 7.2.
• Sequence Positions: Asp18-His259
• Sequence Length: 237

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fusion Tag: C-Fc-6His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for Siglec-3/CD33 recombinant protein

CD33 is a type I Lectin belonging to the Ig superfamily. CD33 contains an N terminal Ig like V type domain, which mediates sialic acid binding, followed by one Ig like C2 type domain, a transmembrane region and a cytoplasmic tail containing two conserved immunoreceptor tyrosine based inhibition motifs (ITIMs). Eleven human Siglecs have been characterized. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec3 both in their extracellular and intracellular regions. They are collectively referred to as CD33 related Siglecs. CD33 related Siglecs have differential expression pattern within the hematopoietic system. They are involved in the regulation of cellular activation within the immune system. Siglec 3 expression is restricted to cells of myelomonocytic lineage. Siglec3 recruits SHP1 and SHP2 to its ITIMs upon phosphorylation.

NCBI and Uniprot Product Information

• NCBI GI #: 130980072
• NCBI GeneID: 945
• NCBI Accession #: NP_001076087.1
• NCBI GenBank Nucleotide #: NM_001082618.1
• UniProt Accession #: P20138; Q8TD24; B4E3P8; C9JEN7; F8WAL2
• Molecular Weight: Molecular Mass: 55.0 kDa; Actual Protein Molecular Mass: 80 kDa
• NCBI Official Full Name: myeloid cell surface antigen CD33 isoform 2
• NCBI Official Synonym Full Names: CD33 molecule
• NCBI Official Symbol: CD33
• NCBI Official Synonym Symbols: p67; SIGLEC3; SIGLEC-3
• NCBI Protein Information: myeloid cell surface antigen CD33
• UniProt Protein Name: Myeloid cell surface antigen CD33
• UniProt Gene Name: CD33
• UniProt Synonym Gene Names: SIGLEC3; Siglec-3

Uniprot Description

Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).