Siglec-2 / CD22 Recombinant Protein | CD22 recombinant protein

Recombinant Mouse Siglec-2 / CD22 Protein (His tag)

Cat. Num. AAA2546632

• Gene Names: Cd22; Lyb8; Lyb-8; A530093D23
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Siglec-2 / CD22; Recombinant Mouse Siglec-2 / CD22 Protein (His tag); CD22; Lyb-8; Siglec2; CD22 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 868

• Application Notes: The recombinant mouse CD22 comprises 701 amino acids and has a predicted molecular mass of 78.8 kDa. The apparent molecular mass of the protein is approximately 116 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Arg 19
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD22 recombinant protein

Background: CD22 is a member of the immunoglobulin superfamily, SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions, signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow, blood, and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. As an inhibitory coreceptor of the B-cell receptor (BCR), CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors, the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR, and this in turn is governed by the binding of its extracellular domain to alpha2, 6-linked sialic acid ligands. However, genetic studies in mice reveal that some CD22 functions are regulated by ligand binding, whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1.

Description: A DNA sequence encoding the mouse CD22 (BAE33829.1) (Met1-Arg708) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 74140264
• NCBI GeneID: 12483
• Molecular Weight: 92,857 Da
• NCBI Official Full Name: unnamed protein product
• NCBI Official Synonym Full Names: CD22 antigen
• NCBI Official Symbol: Cd22
• NCBI Official Synonym Symbols: Lyb8; Lyb-8; A530093D23
• NCBI Protein Information: B-cell receptor CD22
• UniProt Protein Name: B-cell receptor CD22
• Protein Family: B-cell receptor
• UniProt Gene Name: Cd22
• UniProt Synonym Gene Names: Lyb-8; Siglec2; BL-CAM; Siglec-2

Uniprot Description

CD22: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7 B1|7 19.26 cM

Cellular Component: external side of plasma membrane; integral to plasma membrane

Molecular Function: coreceptor activity

Biological Process: cell surface receptor linked signal transduction