CD320 recombinant protein

Recombinant Human CD320 Protein

Cat. Num. AAA8248715

• Gene Names: CD320; 8D6; 8D6A; TCBLR
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: CD320; Recombinant Human CD320 Protein; 8D6A; CD320 antigen; 8D6 antigen; FDC-signaling molecule 8D6; FDC-SM-8D6; Transcobalamin receptor; TCblR; CD320 recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 10mM Tris-Citrate, 150mM NaCl, pH 7.4.
• Sequence: SPLSTPTSAQ AAGPSSGSCP PTKFQCRTSG LCVPLTWRCD RDLDCSDGSD EEECRIEPCT QKGQCPPPPG LPCPCTGVSD CSGGTDKKLR NCSRLACLAG ELRCTLSDDC IPLTWRCDGH PDCPDSSDEL GCGTNEILPE GDATTMGPPV TLESVTSLRN ATTMGPPVTL ESVPSVGNAT SSSAGDQSGS PTAYGVVDHH HHHH
• Sequence Length: 240

• Applicable Applications for CD320 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for CD320 recombinant protein

Recombinant Human CD320 Protein is produced by our mammalian expression system and the target gene encoding Ser36-Val231 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 259906403
• NCBI GeneID: 51293
• NCBI Accession #: NP_001159367.1
• NCBI GenBank Nucleotide #: NM_001165895.1
• UniProt Accession #: Q9NPF0; Q53HF7; B2RDS5; D6W668; F5H6D3
• Molecular Weight: 24,371 Da
• NCBI Official Full Name: CD320 antigen isoform 2
• NCBI Official Synonym Full Names: CD320 molecule
• NCBI Official Symbol: CD320
• NCBI Official Synonym Symbols: 8D6; 8D6A; TCBLR
• NCBI Protein Information: CD320 antigen
• UniProt Protein Name: CD320 antigen
• Protein Family: CD320 antigen
• UniProt Gene Name: CD320
• UniProt Synonym Gene Names: 8D6A; FDC-SM-8D6; TCblR
• UniProt Entry Name: CD320_HUMAN

NCBI Description

This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin (vitamin B12), and is involved in B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]

Uniprot Description

CD320: Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC); also called methylmalonic aciduria due to transcobalamin receptor defect. MMATC is a metabolic disorder characterized by increased blood C3- acylcarnitine levels, elevated methylmalonate and homocysteine, and low uptake of transcobalamin-bound cobalamin, but normal conversion to adenosylcobalamin and methylcobalamin. Plasma vitamin B12 and total homocysteine are normal.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: endoplasmic reticulum; endosome membrane; membrane; plasma membrane

Molecular Function: cobalamin binding; cobalamin transporter activity

Biological Process: cobalamin metabolic process

Disease: Methylmalonic Aciduria Due To Transcobalamin Receptor Defect