Mouse Cyclin-E2 ELISA Kit | CCNE2 elisa kit

Mouse CCNE2 (Cyclin-E2) ELISA Kit

Cat. Num. AAA2533478

• Gene Names: CCNE2; CYCE2
• Reactivity: Mouse
• Synonyms: Cyclin-E2; Mouse CCNE2 (Cyclin-E2) ELISA Kit; CCNE2 elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes Mouse CCNE2 in samples. No significant cross-reactivity or interference between Mouse CCNE2 and analogues was observed.
• Sequence Length: 404

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 18.75pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse CCNE2 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse CCNE2 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCNE2 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse CCNE2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CCNE2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CCNE2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CCNE2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CCNE2. You can calculate the concentration of Mouse CCNE2 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4008085
• NCBI GeneID: 9134
• Molecular Weight: 41,465 Da
• NCBI Official Full Name: cyclin E2
• NCBI Official Synonym Full Names: cyclin E2
• NCBI Official Symbol: CCNE2
• NCBI Official Synonym Symbols: CYCE2
• NCBI Protein Information: G1/S-specific cyclin-E2
• UniProt Protein Name: G1/S-specific cyclin-E2
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: CCNE2
• UniProt Entry Name: CCNE2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNE2: Essential for the control of the cell cycle at the late G1 and early S phase. Interacts with the CDK2 (in vivo) and CDK3 (in vitro) protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Activated by papilloma viral oncoproteins E6 and E7 which bind to and inactivate p53 and Rb, respectively. According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor- derived cells compared to non-transformed proliferating cells. According to PubMed:9840927: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to PubMed:9840943 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon. Belongs to the cyclin family. Cyclin E subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Activator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: cytosol; nucleoplasm

Molecular Function: cyclin-dependent protein kinase regulator activity; protein binding; protein kinase binding

Biological Process: cell cycle checkpoint; cell division; DNA replication initiation; G1/S transition of mitotic cell cycle; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity