Western Blot (WB)
(Western Blot analysis of NCI-H460 cell line lysates (35ug/lane) usingMBS6011657. VTN (arrow) was detected usingMBS6011657.)
Rabbit anti-Human VTN Polyclonal Antibody | anti-VTN antibody
VTN, NT (VTN, Vitronectin, S-protein, Serum-spreading factor, V75, Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B) (MaxLight 490)
Western Blot (WB)
(Western Blot analysis of NCI-H460 cell line lysates (35ug/lane) usingMBS6011657. VTN (arrow) was detected usingMBS6011657.)
Western Blot (WB)
(Western Blot analysis of NCI-H460 cell line lysates (35ug/lane) usingMBS6011657. VTN (arrow) was detected usingMBS6011657.)
Immunohistochemistry (IHC)
(Immunohistochemistry in formalin-fixed and paraffin-embedded human lung carcinoma reacted withMBS6011657, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemistry in formalin-fixed and paraffin-embedded human lung carcinoma reacted withMBS6011657, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
Western Blot (WB)
(Western Blot analysis in human plasma lysate (20ug) using MBS6011657 (1:32,000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 54kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in human plasma lysate (20ug) using MBS6011657 (1:32,000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 54kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data
(Overlay histogram showing MCF-7 cells stained with MBS6011657 (green line). The cells were fixed with 2% paraformaldehyde (10mins) and then permeabilized with 90% methanol for 10mins. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by MBS6011657 (1:25) for 60mins at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:200 for 40mins at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Testing Data
(Overlay histogram showing MCF-7 cells stained with MBS6011657 (green line). The cells were fixed with 2% paraformaldehyde (10mins) and then permeabilized with 90% methanol for 10mins. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by MBS6011657 (1:25) for 60mins at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:200 for 40mins at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10,000 events was performed.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008]
Uniprot Description
VTN: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Cell adhesion
Chromosomal Location of Human Ortholog: 17q11
Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region
Molecular Function: heparin binding; integrin binding; protein binding; extracellular matrix binding; scavenger receptor activity; polysaccharide binding
Biological Process: receptor-mediated endocytosis; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell-matrix adhesion; negative regulation of blood coagulation; positive regulation of smooth muscle cell migration; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of receptor-mediated endocytosis; positive regulation of peptidyl-tyrosine phosphorylation; regulation of complement activation; cell adhesion mediated by integrin; innate immune response; immune response; cell adhesion
Research Articles on VTN
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Product Notes
The VTN vtn (Catalog #AAA6362934) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VTN, NT (VTN, Vitronectin, S-protein, Serum-spreading factor, V75, Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B) (MaxLight 490) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VTN can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB), Flow Cytometry (FC/FACS). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the VTN vtn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VTN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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