anti-Human, Mouse Cyclin E1 Antibody | anti-CCNE1 antibody

Cyclin E1 (Phospho-Thr77) Antibody

Cat. Num. AAA8509547

• Gene Names: CCNE1; CCNE; pCCNE1
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: Cyclin E1; Cyclin E1 (Phospho-Thr77) Antibody; CCNE; G1/S-specific cyclin E1; cyclin E1; anti-CCNE1 antibody

• Reactivity: Human, Mouse
• Specificity: Cyclin E1 (Phospho-Thr77) antibody detects endogenous levels of Cyclin E1 only when phosphorylated at threonine 77.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 410

• Applicable Applications for anti-CCNE1 antibody: Immunofluorescence (IF), ELISA (EIA)
• Application Notes: IF: 1:100-1:500; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Cyclin E1 around the phosphorylation site of threonine 77 (I-P-TP-P-D).
• All Sites: Human: Thr77; Mouse: Thr74
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunofluorescence (IF)

(P-peptide-+ Immunofluorescence analysis of HeLa cells, using Cyclin E1 (Phospho-Thr77) antibody.)

References

Koff A., Cell 66:1217-1228(1991). The MGC Project Team, Genome Res. 14:2121-2127(2004). Geng Y., Oncogene 12:1173-1180(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 17318559
• NCBI GeneID: 898
• NCBI Accession #: NP_001229.1
• NCBI GenBank Nucleotide #: NM_001238.3
• UniProt Accession #: P24864; Q14091; Q8NFG1; Q92501; Q9UD21; A8K684
• Molecular Weight: 45,150 Da
• NCBI Official Full Name: G1/S-specific cyclin-E1 isoform 1
• NCBI Official Synonym Full Names: cyclin E1
• NCBI Official Symbol: CCNE1
• NCBI Official Synonym Symbols: CCNE; pCCNE1
• NCBI Protein Information: G1/S-specific cyclin-E1
• UniProt Protein Name: G1/S-specific cyclin-E1
• UniProt Gene Name: CCNE1
• UniProt Synonym Gene Names: CCNE

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]

Uniprot Description

Essential for the control of the cell cycle at the G1/S (start) transition.