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Human chemokine (C-C motif) ligand 8 ELISA Kit | CCL8 elisa kit

Human monocyte chemotactic protein 2, MCP-2 ELISA Kit

Gene Names
CCL8; HC14; MCP2; MCP-2; SCYA8; SCYA10
Reactivity
Human
Synonyms
chemokine (C-C motif) ligand 8; Human monocyte chemotactic protein 2; MCP-2 ELISA Kit; MCP-2 ELISA kit; HC14; MCP-2; MCP2; SCYA10; SCYA8; monocyte chemoattractant protein 2; monocyte chemotactic protein 2; small inducible cytokine A8; small inducible cytokine subfamily A (Cys-Cys); member 8 (monocyte che; CCL8 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human CCL8. No significant cross-reactivity or interference between Human CCL8 and analogues was observed.
Sequence Length
99
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Immunity
Protein Biological Process 2
chemokine
Protein Biological Process 3
Chemotaxis
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for CCL8 elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MCP-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for MCP-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MCP-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MCP-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11,246 Da
NCBI Official Full Name
C-C motif chemokine 8
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 8
NCBI Official Symbol
CCL8
NCBI Official Synonym Symbols
HC14; MCP2; MCP-2; SCYA8; SCYA10
NCBI Protein Information
C-C motif chemokine 8; small-inducible cytokine A8; monocyte chemotactic protein 2; monocyte chemoattractant protein 2; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2)
UniProt Protein Name
C-C motif chemokine 8
Protein Family
UniProt Gene Name
CCL8
UniProt Synonym Gene Names
MCP2; SCYA10; SCYA8; MCP-2
UniProt Entry Name
CCL8_HUMAN

NCBI Description

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. [provided by RefSeq, Jul 2013]

Uniprot Description

Function: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.1 PublicationManual assertion based on experiment in:Ref.7

Research Articles on CCL8

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Product Notes

The Human CCL8 ccl8 (Catalog #AAA702780) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA702780 ELISA Kit recognizes Human CCL8. It is sometimes possible for the material contained within the vial of "chemokine (C-C motif) ligand 8, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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