TECK (CCL25) Recombinant Protein | CCL25 recombinant protein
Mouse TECK (CCL25) Recombinant
Purity
>97%, as determined by SDS-PAGE and HPLC
Synonyms
TECK (CCL25); Mouse TECK (CCL25) Recombinant; Mouse TECK Recombinant; teck; ccl25; SCYA25; CCL25 recombinant protein
Host
E Coli
Purity/Purification
>97%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant CCL25 was was lyophilized from 0.2um filtered PBS, pH 7.4.
Sequence
MKLWLFACLV ACFVGAWMPV VHAQGAFEDC CLGYQHRIKW NVLRHARNYH QQEVSGSCNL RAVRFYFRQK VVCGNPEDMN VKRAIRILTA RKRLVHWKSA SDSQTERKKS NHMKSKVENP NSTSVRSATL GHPRMVMMPR KTNN
Sequence Length
144
Domain
IL8
Host Note
Optimized DNA sequence encoding mouse CCL25 mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native mouse CCL25 has a calculated molecular mass of approximately 14 kDa.
Recombinant CCL25 is a disulfide-linked homodimeric protein consisting of 128 amino acid residue subunits, and migrates as an approximately 14 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Recombinant CCL25 is a disulfide-linked homodimeric protein consisting of 128 amino acid residue subunits, and migrates as an approximately 14 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Preparation and Storage
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Related Product Information for CCL25 recombinant protein
CCL25 is constitutively expressed in epithelium and mucosal vessels in the small bowel, where it interacts with gut-homing B and T cells expressing its receptor, CCR9.
Product Categories/Family for CCL25 recombinant protein
References
• angiotensin-converting enzyme inhibition down-regulates the pro-atherogenic chemokine receptor 9 (ccr9) -chemokine ligand 25 (CCL25) axis .J. Biol. Chem., Jul 2010; 285: 23496 - 23505.
• thymic progenitor homing and lymphocyte homeostasis are linked via s1p-controlled expression of thymic p-selectin/CCL25 .J. Exp. Med., Apr 2009; 206: 761 - 778.
• CCL25 increases thymopoiesis after androgen withdrawal .Blood, Oct 2008; 112: 3255 - 3263.
• CCL25 and ccl28 promote 4β7-integrin-dependent adhesion of lymphocytes to madcam-1 under shear flow .Am J Physiol Gastrointest Liver Physiol, May 2008; 294: G1257 - G1267.
• activation of ccr9/CCL25 in cutaneous melanoma mediates preferential metastasis to the small intestine .Clin. Cancer Res., Feb 2008; 14: 638 - 645.
• thymic progenitor homing and lymphocyte homeostasis are linked via s1p-controlled expression of thymic p-selectin/CCL25 .J. Exp. Med., Apr 2009; 206: 761 - 778.
• CCL25 increases thymopoiesis after androgen withdrawal .Blood, Oct 2008; 112: 3255 - 3263.
• CCL25 and ccl28 promote 4β7-integrin-dependent adhesion of lymphocytes to madcam-1 under shear flow .Am J Physiol Gastrointest Liver Physiol, May 2008; 294: G1257 - G1267.
• activation of ccr9/CCL25 in cutaneous melanoma mediates preferential metastasis to the small intestine .Clin. Cancer Res., Feb 2008; 14: 638 - 645.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
16,714 Da
NCBI Official Full Name
C-C motif chemokine 25
UniProt Protein Name
C-C motif chemokine 25
Protein Family
UniProt Gene Name
Ccl25
UniProt Synonym Gene Names
Scya25; Teck
UniProt Entry Name
CCL25_MOUSE
Uniprot Description
CCL25: Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Belongs to the intercrine beta (chemokine CC) family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine; Secreted
Cellular Component: extracellular space; extracellular region
Molecular Function: protein binding; chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding; cytokine activity
Biological Process: G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; positive regulation of cell-matrix adhesion; chemotaxis; leukocyte migration
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Product Notes
The CCL25 ccl25 (Catalog #AAA553239) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MKLWLFACLV ACFVGAWMPV VHAQGAFEDC CLGYQHRIKW NVLRHARNYH QQEVSGSCNL RAVRFYFRQK VVCGNPEDMN VKRAIRILTA RKRLVHWKSA SDSQTERKKS NHMKSKVENP NSTSVRSATL GHPRMVMMPR KTNN. It is sometimes possible for the material contained within the vial of "TECK (CCL25), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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