CCL22 recombinant protein

CCL22

Cat. Num. AAA517291

• Gene Names: CCL22; MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• Applications: Cell Assay
• Synonyms: CCL22; STCP-1; ABCD-1; CC chemokine STCP-1; MDC(1-69); SCYA22; Small-inducible cytokine A22; Stimulated T-cell chemotactic protein 1; MDC; C-C motif chemokine 22; Recombinant Human Macrophage-Derived Chemokine (CCL22).; CCL22 recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from 0.2 um filtered solution in Tris and NaCl, pH7.0.

• Applicable Applications for CCL22 recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
• Preparation and Storage: Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months. Avoid repeated freeze/thaw cycles.

Activity

(Sample Cell Assay Data: Determined by its ability to chemoattract human T cells using a concentration range of 10-50 ng/mL.)

SDS-Page

(Purity: Recombinant Human CCL22 resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.)

Related Product Information for CCL22 recombinant protein

Recombinant Human CCL22 was expressed in E Coli cells. The protein accession number is O00626
CCL22 (monocyte-derived chemokine) was described initially as a constitutively produced, thymus-specific chemokine, implicated in the recruitment of T cells. One of the functions of CCL22 originally described was induction of migration of CCR4+ Th2 cells, but it has additionally been shown to regulate migration of Tregs. CCL22 has been implicated in a number of diseases, including allergen-induced lung inflammation, atopic dermatitis, and lymphoma. Similarly, a presumed role for CCR4+ cells has been suggested for endotoxic shock, rheumatoid arthritis, T cell lymphoma, and autoimmune diabetes.

Product Categories/Family for CCL22 recombinant protein

Growth Factors; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 1931581
• NCBI GeneID: 6367
• UniProt Accession #: O00626; A0N0Q6; B2R4W2
• Molecular Weight: 10,625 Da
• NCBI Official Full Name: macrophage-derived chemokine
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 22
• NCBI Official Symbol: CCL22
• NCBI Official Synonym Symbols: MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• NCBI Protein Information: C-C motif chemokine 22; MDC(1-69); CC chemokine STCP-1; macrophage-derived chemokine; small inducible cytokine A22; small-inducible cytokine A22; stimulated T cell chemotactic protein 1; stimulated T-cell chemotactic protein 1; small inducible cytokine su
• UniProt Protein Name: C-C motif chemokine 22
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL22
• UniProt Synonym Gene Names: MDC; SCYA22
• UniProt Entry Name: CCL22_HUMAN

NCBI Description

This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. The product of this gene binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. [provided by RefSeq, Jul 2008]

Uniprot Description

CCL22: May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity

Biological Process: cell-cell signaling; response to virus; immune response; signal transduction; inflammatory response; chemotaxis