MIP-4 (CCL18) Recombinant Protein | MIP-4 recombinant protein

Human MIP-4 (CCL18) Recombinant

Cat. Num. AAA553104

• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: MIP-4 (CCL18); Human MIP-4 (CCL18) Recombinant; Human MIP4 (CCL18) Recombinant; ccl-18 ; CCL18; mip-4; mip4; AMAC1; DCCK1; PARC; SCYA18); MIP-4 recombinant protein

• Host: E Coli
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant CCL-18 was lyophilized from a 0.2 um filtered 20mM PB,100mM NaCl solution pH 7.5.
• Sequence: MKGLAAALLV LVCTMALCSC AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA
• Sequence Length: 89

• Domain: IL8
• Host Note: Optimized DNA sequence encoding Human MIP-4 (CCL18) mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Human CCL-18, generated by the proteolytic removal of the signal peptide; and propeptide and has a calculated molecular mass of approximately 8kDa.; ; Recombinant CCL18 is a disulfide-linked homodimeric protein consisting of 70 amino acid residue subunits and migrates as an approximately 16 kDa protein under non-reducing conditions and 8kDa under reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for MIP-4 recombinant protein

CCL18, also designated dendritic cell (DC) 3-derived CC chemokine 1, pulmonary and activation-regulated chemokine, alternative macrophage activation- associated CC chemokine 1, and MIP-4, is a human chemokine structurally related to CCL3.CCL18 was shown to be expressed in germinal centers of tonsils by dendritic cells and to attract mainly naive T cells, CD38- mantle zone B lymphocytes and DC. The production of CCL18 by APC is enhanced by Th2 cytokines like IL-4 and IL-13 and suppressed by IFN-gamma.

Product Categories/Family for MIP-4 recombinant protein

Cytokines

References

• gpr30 expression on pre-b acute lymphocytic leukemia cells results in modulation of cxcr4 activity by CCL18. .Blood (ASH Annual Meeting Abstracts), Nov 2009; 114: 2671.
• protein microarray analysis in patients with asthma: elevation of the chemokine parc/CCL18 in sputum .Chest, Feb 2009; 135: 295 - 302.
• mature dendritic cells express functional thrombin receptors triggering chemotaxis and CCL18/pulmonary and activation-regulated chemokine induction .J. Immunol., Jul 2008; 181: 1215 - 1223.
• cc chemokine ligand-5 (ccl5/rantes) and cc chemokine ligand-18 (CCL18/parc) are specific markers of refractory unstable angina pectoris and are transiently raised during severe ischemic symptoms .Circulation, Oct 2007; 116: 1931 - 1941.
• circulating levels of the chemokine CCL18 but not cxcl16 are elevated and correlate with disease activity in rheumatoid arthritis .Ann Rheum Dis, Oct 2007; 66: 1334 - 1338.