MIP-4 (CCL18) Recombinant Protein | MIP-4 recombinant protein

MIP-4 (CCL18)

Cat. Num. AAA517299

• Gene Names: CCL18; CKb7; PARC; AMAC1; DCCK1; MIP-4; AMAC-1; DC-CK1; SCYA18
• Applications: Cell Assay
• Synonyms: MIP-4 (CCL18); MIP4; CCL18; Pulmonary and activation-regulated chemokine; PARC; DC-CK1; AMAC-1; C-C motif chemokine 18; Small-inducible cytokine A18; Recombinant Human Macrophage Inflammatory Protein-4 (CCL18); MIP-4 recombinant protein

• Host: E Coli
• Form/Format: Recombinant CC18 was lyophilized from a 0.2 um filtered 20mM PB,100mM NaCl solution pH7.5.

• Applicable Applications for MIP-4 recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method.
• Sample Cell Assay Data: Determined by its ability to chemoattract human T cells using a concentration range of 2-40ng/mL.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

SDS-Page

(Purity: Recombinant Human MIP-4 (CCL18) resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250)

Related Product Information for MIP-4 recombinant protein

Recombinant Human MIP-4 (CCL18) was expressed in E Coli cells. The protein accession number is P55774
CCL18, also designated dendritic cell (DC)3-derived CC chemokine 1, pulmonary and activation-regulated chemokine, alternative macrophage activation-associated CC chemokine 1, and MIP-4, is a human chemokine structurally related to CCL3. CCL18 was shown to be expressed in germinal centers of tonsils by dendritic cells and to attract mainly naive T cells, CD38 mantle zone B lymphocytes and DC. The production of CCL18 by APC is enhanced by Th2 cytokines like IL-4 and IL-13 and suppressed by IFN-gamma.

Product Categories/Family for MIP-4 recombinant protein

Growth Factors; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4836258
• NCBI GeneID: 6362
• UniProt Accession #: P55774; Q53X71; B5BUM2
• Molecular Weight: 9,849 Da
• NCBI Official Full Name: alternative macrophage activation-associated CC-chemokine
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
• NCBI Official Symbol: CCL18
• NCBI Official Synonym Symbols: CKb7; PARC; AMAC1; DCCK1; MIP-4; AMAC-1; DC-CK1; SCYA18
• NCBI Protein Information: C-C motif chemokine 18; CC chemokine PARC; CC chemokine ligand 18; chemokine (C-C), dendritic; dendritic cell chemokine 1; small inducible cytokine A18; small-inducible cytokine A18; macrophage inflammatory protein 4; pulmonary and activation-regulated ch
• UniProt Protein Name: C-C motif chemokine 18
• UniProt Gene Name: CCL18
• UniProt Synonym Gene Names: AMAC1; DCCK1; MIP4; PARC; SCYA18; AMAC-1; DC-CK1; MIP-4
• UniProt Entry Name: CCL18_HUMAN

NCBI Description

This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for naive T cells, CD4+ and CD8+ T cells and nonactivated lymphocytes, but not for monocytes or granulocytes. This chemokine attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes. It may play a role in both humoral and cell-mediated immunity responses. [provided by RefSeq, Jul 2008]

Uniprot Description

CCL18: Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Chemokine

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function: protein binding; chemokine activity

Biological Process: cell-cell signaling; response to biotic stimulus; immune response; cell communication; chemotaxis; signal transduction; inflammatory response