CCL17 recombinant protein

Recombinant Human CCL17

Cat. Num. AAA226084

• Gene Names: CCL17; TARC; ABCD-2; SCYA17; A-152E5.3
• Applications: ELISA
• Purity: Recombinant protein expressed in E Coli and purified by ion exchange chromatography; >=90 % determined by coomassie blue staining of a reducing SDS-PAGE gel
• Synonyms: CCL17; Recombinant Human CCL17; CCL17 recombinant protein

• Host: E Coli
• Purity/Purification: Recombinant protein expressed in E Coli and purified by ion exchange chromatography; >=90 % determined by coomassie blue staining of a reducing SDS-PAGE gel
• Form/Format: 50 mM Tris-HCl, pH 8.0, 0.15 M Sodium Chloride; 1.0% Trehalose; Purified recombinant protein - lyophilized
• Concentration: 100ug/ml after reconstitution (varies by lot)

• Applicable Applications for CCL17 recombinant protein: ELISA (EIA)
• Target Species: Human
• Reconstitution: Centrifuge vial prior to reconstitution. Reconstitute to 100ug/ml by adding 100ul ddH2O.; ; Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Lab recommends that the vial is gently mixed after reconstitution. Do not vortex.
• Preparation and Storage: Prior to reconstitution store at -70 degree C. Following reconstitution store at -80 degree C.; ; This product should be stored undiluted.; ; Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

SDS-Page

(SDS-PAGE analysis of recombinant Human CCL17Recombinant Human CCL17 (MBS226084) was analyzed under reducing denaturing conditions (Lane 2). The molecular weight (kDa) of the marker proteins (Lane 1) are displayed on the left hand of the image. Bands )

Related Product Information for CCL17 recombinant protein

Recombinant Human CCL17

CCL17 also known as TARC and SCYA17 is a C-C chemokine highly expressed by the thymus and other cell types, including fibroblasts (Li et al. 2019). CCL17 induces and enhances a broad spectrum of immune reactions, including some contact hypersensitivity reactions, allograft rejections, inflammatory bowel disease, atopic dermatitis and atherosclerosis (Scheu et al. 2017). Therapeutic use of antibodies to neutralize CCL17 can block influx of T cells and ameliorate the impact of various diseases (Teplyakov et al. 2017). Upregulation of CCL17 has been reported in breast cancer, and predicts a decrease in overall survival for patients with breast cancer (Li et al. 2019).

This recombinant Human CCL17 can be used to create a standard curve in a sandwich ELISA with purified Mouse anti Human CCL17 antibody, clone I02-2A4 as the capture antibody and biotinylated Mouse anti Human CCL17 antibody, clone I11-2A1 as the detection antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 3024711
• NCBI GeneID: 6361
• UniProt Accession #: Q92583; Q2M287; A0N0Q9
• Molecular Weight: 94
• NCBI Official Full Name: C-C motif chemokine 17
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 17
• NCBI Official Symbol: CCL17
• NCBI Official Synonym Symbols: TARC; ABCD-2; SCYA17; A-152E5.3
• NCBI Protein Information: C-C motif chemokine 17; CC chemokine TARC; T cell-directed CC chemokine; small-inducible cytokine A17; thymus and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 17
• UniProt Protein Name: C-C motif chemokine 17
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL17
• UniProt Synonym Gene Names: SCYA17; TARC
• UniProt Entry Name: CCL17_HUMAN

NCBI Description

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. The product of this gene binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL17: Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; extracellular region

Molecular Function: CCR4 chemokine receptor binding; chemokine activity; receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; multicellular organismal development; immune response; negative regulation of myoblast differentiation; inflammatory response; chemotaxis