Human CCL17/TARC ELISA Kit | CCL17 elisa kit

Human CCL17/TARC PicoKine ELISA Kit

Cat. Num. AAA175885

• Gene Names: CCL17; TARC; ABCD-2; SCYA17; A-152E5.3
• Reactivity: Human
• Synonyms: CCL17/TARC; Human CCL17/TARC PicoKine ELISA Kit; C-C motif chemokine 17; chemokine (C-C motif) ligand 17; A 152E5.3; ABCD 2; C C motif chemokine 17; CC chemokine TARC; CCL 17; CCL17; CCL17_HUMAN; Chemokine (C C motif) ligand 17; Chemokine ligand 17; MGC138271; MGC138273; SCYA17; Small inducible cytokine A17; Small inducible cytokine subfamily A (Cys Cys) member 17; Small inducible cytokine subfamily A member 17; Small-inducible cytokine A17; T cell directed CC chemokine; Thymus and activation regulated chemokine; Thymus and activation-regulated chemokine; CCL17 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CCL17
• Sequence Length: 94

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 1pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human CCL17/TARC PicoKine ELISA Kit standard curve )

Related Product Information for CCL17 elisa kit

Principle of the assay: human CCL17 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL17 has been precoated onto 96-well plates. Standards (E.coli,A24-S94) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL17 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine (TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically bindsand induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2The genefor CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.3,4 Thestandard used in this kit is recombinant human CCL17, consisting of 71 amino acids with the molecular weight of 8Kda.

NCBI and Uniprot Product Information

• NCBI GI #: 3024711
• NCBI GeneID: 6361
• UniProt Accession #: Q92583; Q2M287; A0N0Q9
• Molecular Weight: 10,507 Da
• NCBI Official Full Name: C-C motif chemokine 17
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 17
• NCBI Official Symbol: CCL17
• NCBI Official Synonym Symbols: TARC; ABCD-2; SCYA17; A-152E5.3
• NCBI Protein Information: C-C motif chemokine 17; CC chemokine TARC; T cell-directed CC chemokine; small-inducible cytokine A17; thymus and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 17
• UniProt Protein Name: C-C motif chemokine 17
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL17
• UniProt Synonym Gene Names: SCYA17; TARC
• UniProt Entry Name: CCL17_HUMAN

NCBI Description

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. The product of this gene binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL17: Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; extracellular region

Molecular Function: CCR4 chemokine receptor binding; chemokine activity; receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; multicellular organismal development; immune response; chemotaxis; negative regulation of myoblast differentiation; inflammatory response