CCL14 recombinant protein

CCL14 Protein, Human, Recombinant (aa 28-93, His Tag)

Cat. Num. AAA8120299

• Gene Names: CCL14; CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3
• Purity: >96% as determined by SDS-PAGE
• Synonyms: CCL14; CCL14 Protein; Human; Recombinant (aa 28-93; His Tag); Human CCL14/HCC-1/HCC-3 Protein (aa 28-93; CC-1 Protein; CC-3 Protein; CKB1 Protein; FLJ16015 Protein; HCC-1 Protein; HCC-1(1-74) Protein; HCC-1/HCC-3 Protein; HCC-3 Protein; MCIF Protein; NCC-2 Protein; NCC2 Protein; SCYA14 Protein; SCYL2 Protein; SY14 Protein; chemokine (C-C motif) ligand 14; CCL14 recombinant protein

• Host: E Coli
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly28-Asn93

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the amino acids (Gly 28-Asn 93) of human CCL14 (Q16627-1) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CCL14 recombinant protein

Background: CCL14 has an important biological role in other mammals by evolving under positive selection that has been lost in Ochotonidae (subgenera Pika and Lagotona). CC chemokine ligand 14, CCL14, is a human CC chemokine that is of recent interest because of its natural ability, upon proteolytic processing of the first eight NH2-terminal residues, to bind to and signal through the human immunodeficiency virus type-1 (HIV-1) co-receptor, CC chemokine receptor 5 (CCR5). Embryo implantation is a complex process involving blastocyst attachment to the endometrial epithelium and subsequent trophoblast invasion of the decidua. We have previously shown that the chemokines CX3CL1 and CCL14 are abundant in endometrial vasculature, epithelial, and decidual cells at this time, and that their receptors, CX3CR1 and CCR1, are present on invading human trophoblasts. CX3CL1 and CCL14 promote trophoblast migration.

References

Laing KJ, et al. (2004) Chemokines. Developmental and comparative immunology. 28(5): 443-60.Knappe S, et al. (1996) HCC-1, a novel chemokine from human plasma. J Exp Med. 183: 295-9.Naruse, et al. (1996) A YAC contig of the human CC chemokine genes clustered on chromosome 17q11.2. Genomics. 34: 236-40.

NCBI and Uniprot Product Information

• NCBI GI #: 14589961
• NCBI GeneID: 6358
• NCBI Accession #: NP_116739.1
• NCBI GenBank Nucleotide #: NM_032963.3
• UniProt Accession #: Q16627; Q13954; E1P649; E1P650
• Molecular Weight: 10,678 Da
• NCBI Official Full Name: C-C motif chemokine 14 isoform 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 14
• NCBI Official Symbol: CCL14
• NCBI Official Synonym Symbols: CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3
• NCBI Protein Information: C-C motif chemokine 14; chemokine CC-3; new CC chemokine 2; chemokine CC-1/CC-3; hemofiltrate CC chemokine 1; small-inducible cytokine A14; small inducible cytokine subfamily A (Cys-Cys), member 14
• UniProt Protein Name: C-C motif chemokine 14
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL14
• UniProt Synonym Gene Names: NCC2; SCYA14; HCC-1/HCC-3
• UniProt Entry Name: CCL14_HUMAN

NCBI Description

This gene, chemokine (C-C motif) ligand 14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Read-through transcripts are also expressed that include exons from the upstream cytokine gene, chemokine (C-C motif) ligand 15, and are represented as GeneID: 348249. [provided by RefSeq, Dec 2009]

Uniprot Description

CCL14: Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function: chemokine activity

Biological Process: cellular calcium ion homeostasis; positive regulation of cell proliferation; immune response