HCC-1 (CCL14) Recombinant Protein | HCC-1 recombinant protein

Human HCC-1 (CCL14) Recombinant

Cat. Num. AAA553001

• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: HCC-1 (CCL14); Human HCC-1 (CCL14) Recombinant; ccl-14 ; CCL14; hcc-1; hcc1; HCC-1 recombinant protein

• Host: E Coli
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant HCC-1 was lyophilized from a 0.2 um filtered 20mM PB,100mM NaCl solution pH 7.5.
• Sequence: MKISVAAIPF FLLITIALGT KTESSSRGPY HPSECCFTYT TYKIPRQRIM DYYETNSQCS KPGIVFITKR GHSVCTNPSD KWVQDYIKDM KEN
• Sequence Length: 109

• Domain: IL8
• Host Note: Optimized DNA sequence encoding Human HCC-1 (CCL14) mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Human HCC-1, generated by the proteolytic removal of the signal peptide; and propeptide and has a calculated molecular mass of approximately 9 kDa.; ; Recombinant HCC-1 is a disulfide-linked monomeric protein consisting of 73 amino acid residue subunits and migrates as an approximately 9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for HCC-1 recombinant protein

Hemofiltrate C-C chemokine (HCC) -1 is a recently described monocyte chemoattractant. CCL14 (also known as HCC-1) belongs to the CC chemokine family. Its mature propeptide is a low- affinity agonist of CCR1 that is converted to a high- affinity agonist of CCR1 and CCR5 on proteolytic processing by serine proteases. Determination of the amino acid sequence of HCC-1 revealed four cysteine residues in positions characteristic of the C-C chemokine family, and comparison with the sequences of other chemokines revealed that HCC-1 was most homologous to MIP-1a. However, several functional properties of HCC-1 were atypical of chemokines. Unlike other chemokines, HCC-1 was expressed constitutively in a number of tissues and was present at high concentrations in normal human plasma. In addition, HCC-1 was reported not to be chemotactic for leukocytes.

Product Categories/Family for HCC-1 recombinant protein

Cytokines

References

• hemofiltrate cc chemokines with unique biochemical properties: HCC-1/ccl14a and hcc-2/ccl15 .J. Leukoc. Biol., Sep 2001; 70: 357.
• identification of c-c chemokine receptor 1 (ccr1) as the monocyte hemofiltrate c-c chemokine (hcc) -1 receptor .J. Exp. Med., Aug 1998; 188: 603 - 608.