HCC-1 (CCL14) Recombinant Protein | HCC-1 recombinant protein

HCC-1 (CCL14)

Cat. Num. AAA517061

• Gene Names: CCL14; CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3
• Applications: Cell Assay
• Synonyms: HCC-1 (CCL14); C-C motif chemokine 14; Chemokine CC-1; HCC1; HCC-3; HCC-1(1-74); NCC-2; Small-inducible cytokine A14; CCL14; NCC2; SCYA14; HCC-1 recombinant protein

• Host: E Coli
• Form/Format: Recombinant HCC-1 was lyophilized from a 0.2 um filtered 20mM PB,100mM NaCl solution pH7.5.

• Applicable Applications for HCC-1 recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Activity

(Sample Cell Assay Dat: Determined by its ability to chemoattract human monocytes using a concentration range of 2-40 ng/mL.)

SDS-Page

(Purity: Recombinant Human HCC-1 (CCL14) resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250)

Related Product Information for HCC-1 recombinant protein

Recombinant Human HCC-1 (CCL14) was expressed in E Coli cells. The protein accession number is Q16627
Hemofiltrate C-C chemokine (HCC)-1 is a recently described monocyte chemoattractant. CCL14 (also known as HCC-1) belongs to the CC chemokine family. Its mature propeptide is a low-affinity agonist of CCR1 that is converted to a high-affinity agonist of CCR1 and CCR5 on proteolytic processing by serine proteases. Determination of the amino acid sequence of HCC-1 revealed four cysteine residues in positions characteristic of the C-C chemokine family, and comparison with the sequences of other chemokines revealed that HCC-1 was most homologous to MIP-1a. However, several functional properties of HCC-1 were atypical of chemokines. Unlike other chemokines, HCC-1 was expressed constitutively in a number of tissues and was present at high concentrations in normal human plasma. In addition, HCC-1 was reported not to be chemotactic for leukocytes.

Product Categories/Family for HCC-1 recombinant protein

Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 14589961
• NCBI GeneID: 6358
• NCBI Accession #: NP_116739.1
• NCBI GenBank Nucleotide #: NM_032963.3
• UniProt Accession #: Q16627; Q13954; E1P649; E1P650
• Molecular Weight: 10,678 Da
• NCBI Official Full Name: C-C motif chemokine 14 isoform 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 14
• NCBI Official Symbol: CCL14
• NCBI Official Synonym Symbols: CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3
• NCBI Protein Information: C-C motif chemokine 14; chemokine CC-3; new CC chemokine 2; chemokine CC-1/CC-3; hemofiltrate CC chemokine 1; small-inducible cytokine A14; small inducible cytokine subfamily A (Cys-Cys), member 14
• UniProt Protein Name: C-C motif chemokine 14
• UniProt Gene Name: CCL14
• UniProt Synonym Gene Names: NCC2; SCYA14; HCC-1/HCC-3
• UniProt Entry Name: CCL14_HUMAN

NCBI Description

This gene, chemokine (C-C motif) ligand 14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Read-through transcripts are also expressed that include exons from the upstream cytokine gene, chemokine (C-C motif) ligand 15, and are represented as GeneID: 348249. [provided by RefSeq, Dec 2009]

Uniprot Description

CCL14: Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function: chemokine activity

Biological Process: cellular calcium ion homeostasis; positive regulation of cell proliferation; immune response