Carbonyl Reductase 3 Recombinant Protein | CBR3 recombinant protein

Recombinant Carbonyl Reductase 3 (CBR3)

Cat. Num. AAA2028923

• Gene Names: CBR3; hCBR3; SDR21C2; HEL-S-25
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Carbonyl Reductase 3; Recombinant Carbonyl Reductase 3 (CBR3); CBR3 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MSSCSRVALV TGANRGIGLA IARELCRQFS GDVVLTARDV ARGQAAVQQLQAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLNVLVNN AAVAFKSDDPMPFDIKAEMT LKTNFFATRN MCNELLPIMK PHGRVVNISS LQCLRAFENCSEDLQERFHS ETLTEGDLVD LMKKFVEDTK NEVHEREGWP NSPYGVSKLGVTVLSRILAR RLDEKRKADR ILVNACCPGP VKTDMDGKDS IRTVEEGAETPVYLALLPPD ATEPQGQLVH DKVVQNW
• Sequence Length: 277

• Applicable Applications for CBR3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 34.6kDa
• Accurate Molecular Mass (KD): 40kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Trp277
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119630152
• NCBI GeneID: 874
• Molecular Weight: 30,850 Da
• NCBI Official Full Name: carbonyl reductase 3
• NCBI Official Synonym Full Names: carbonyl reductase 3
• NCBI Official Symbol: CBR3
• NCBI Official Synonym Symbols: hCBR3; SDR21C2; HEL-S-25
• NCBI Protein Information: carbonyl reductase [NADPH] 3
• UniProt Protein Name: Carbonyl reductase [NADPH] 3
• Protein Family: Carbonyl reductase
• UniProt Gene Name: CBR3
• UniProt Entry Name: CBR3_HUMAN

NCBI Description

Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. [provided by RefSeq, Jul 2008]

Uniprot Description

CBR3: Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro). Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.1.1.184

Chromosomal Location of Human Ortholog: 21q22.2

Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol

Molecular Function: 3-keto sterol reductase activity; carbonyl reductase (NADPH) activity

Biological Process: phylloquinone catabolic process; cognition