ECGFpro2 supplement
Bovine ECGFpro2 (complete for lymphatic ECs)
Reactivity
Human Cells, Mouse Cells, Cattle Cells
Synonyms
ECGFpro2; Bovine ECGFpro2 (complete for lymphatic ECs); Endothelial cell growth factor (ECGF); Endothelial cell growth supplement (ECGS); ECGFpro2 supplement
Host
Cattle
Reactivity
Human Cells, Mouse Cells, Cattle Cells
Form/Format
Crude extract; Lyophilized (freeze-dried)
Species
Bovine
Reconstitution
Reconstitute the contents of the vial in 2 ml of prewarmed (37 degree C) sterile PBS or water. Gently rotate the vial until the contents are dissolved. This stock solution may be further diluted in sterile tissue culture media to obtain the desired working concentrations. It is recommended that medium containing diluted product is aseptically filtered prior to use.
Biological Activity
Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 ug/ml, optimal concentration with heparin (50 ug/ml) is about 12 ug/ml.
Remarks
BSE-free tested!
Preparation and Storage
Also stable at 4 degree C for several weeks it is recommended to store the product below 0 degree C. After reconstitution the product shout be stored in aliquots at -20 degree C to -70 degree C.
Related Product Information for ECGFpro2 supplement
Endothelial cell growth factor (ECGF) is an extract of bovine brain containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth factor is prepared using a modification of the method of Maciag, et al. (1979) lyophilized from a sterile solution containing NaCl and streptomycin sulfate. ECGFpro2 is supplemented with recombinant human VEGF-C (corresponding to 50 ng/ml) a concentration sufficient for the cultivation of lymphatic endothelial cells. Lymphatic endothelial cells from human skin can be established as primary cultures by traditional methods. The serial propagation of these cells has proved to be difficult. The long-term propagation of these cells in vitro can be achieved with an extract prepared from bovine brain. The introduction of a fibronectin or collagen matrix to the cell culture system allows cultivating endothelial cells at clonal densities. With ECGF, the FCS requirement can be reduced. Heparin potentiates the mitogenic activity of crude preparations of ECGF. ECGF has also been reported to eliminate the need for feeder cells in the clonal growth of hybridomas and other cell types.
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Product Notes
The ECGFpro2 (Catalog #AAA692239) is a Supplement produced from Cattle and is intended for research purposes only. The product is available for immediate purchase. The Bovine ECGFpro2 (complete for lymphatic ECs) reacts with Human Cells, Mouse Cells, Cattle Cells and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "ECGFpro2, Supplement" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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