Cat E3 ubiquitin-protein ligase Mdm2 ELISA Kit | MDM2 elisa kit

Cat E3 ubiquitin-protein ligase Mdm2 (MDM2) ELISA Kit

Cat. Num. AAA285237

• Reactivity: Cat
• Synonyms: E3 ubiquitin-protein ligase Mdm2; Cat E3 ubiquitin-protein ligase Mdm2 (MDM2) ELISA Kit; HDMX; MGC5370; MGC71221; hdm2; Mdm2; transformed 3T3 cell double minute 2; p53 binding protein|OTTHUMP00000183495|double minute 2; human homolog of; p53-binding protein|mouse double minute 2 homolog; MDM2 elisa kit

• Reactivity: Cat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cat MDM2. No significant cross-reactivity or interference between Cat MDM2 and analogues was observed.
• Sequence Length: 458

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MDM2 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MDM2 in samples. An antibody specific for MDM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 57163889
• NCBI GeneID: 493939
• NCBI Accession #: NP_001009346.1
• NCBI GenBank Nucleotide #: NM_001009346.1
• UniProt Accession #: Q7YRZ8
• Molecular Weight: 55,433 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase Mdm2
• NCBI Official Symbol: MDM2
• NCBI Protein Information: E3 ubiquitin-protein ligase Mdm2
• UniProt Protein Name: E3 ubiquitin-protein ligase Mdm2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: MDM2

Uniprot Description

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation. Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells. Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis.