Typical Testing Data/Standard Curve (for reference only)
Cattle Glucagon Like Peptide 1 (GLP1) ELISA Kit | GLP1 elisa kit
Cattle Glucagon Like Peptide 1 (GLP1) Mini Samples ELISA Kit
Reactivity
Cattle
Applications
ELISA
Synonyms
Glucagon Like Peptide 1 (GLP1); Cattle Glucagon Like Peptide 1 (GLP1) Mini Samples ELISA Kit; GLP1 elisa kit
Reactivity
Cattle
Specificity
This assay has high sensitivity for detection of GLP1. 100% cross-reactivity of GLP1 was observed among human, mouse, rat, rabbit, bovine and canine.
Applicable Applications for GLP1 elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked Immunosorbent Assay for Antigen Detection.
Samples
Serum, plasma and other biological fluids. due to the 100% homology of the sequence among different species, the kit can be used to detect human, mouse, rat, rabbit, bovine and canine samples
Assay Type
Quantitative Competitive
Detection Range
12.35-1,000pg/mL
Sensitivity
< 4.64pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GLP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GLP1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GLP1 elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of GLP1 in rat serum, plasma and other biological fluids. Due to the 100% homology of the sequence among different species, the kit can be used to detect human, mouse, rat, rabbit, bovine and canine samples.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to GLP1 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled GLP1 and unlabeled GLP1 (Standards or samples) with the pre-coated antibody specific to GLP1. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of GLP1 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of GLP1 in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to GLP1 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled GLP1 and unlabeled GLP1 (Standards or samples) with the pre-coated antibody specific to GLP1. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of GLP1 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of GLP1 in the sample.
Product Categories/Family for GLP1 elisa kit
Similar Products
Product Notes
The Cattle GLP1 (Catalog #AAA2124071) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2124071 ELISA Kit recognizes Cattle GLP1. AAA Biotech's Glucagon Like Peptide 1 (GLP1) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked Immunosorbent Assay for Antigen Detection. Researchers should empirically determine the suitability of the GLP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glucagon Like Peptide 1 (GLP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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