Mouse Caspase-6 ELISA Kit | CASP6 elisa kit

Mouse Caspase-6 (CASP6) ELISA Kit

Cat. Num. AAA281999

• Gene Names: Casp6; Mch2; CASP-6
• Reactivity: Mouse
• Synonyms: Caspase-6; Mouse Caspase-6 (CASP6) ELISA Kit; CASP6 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse CASP6. No significant cross-reactivity or interference between Mouse CASP6 and analogues was observed.
• Sequence Length: 304

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for CASP6 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CASP6 in samples. An antibody specific for CASP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCASP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CASP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CASP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 157951736
• NCBI GeneID: 12368
• NCBI Accession #: NP_033941.3
• NCBI GenBank Nucleotide #: NM_009811.4
• UniProt Accession #: O08738
• Molecular Weight: 31,595 Da
• NCBI Official Full Name: caspase-6
• NCBI Official Synonym Full Names: caspase 6
• NCBI Official Symbol: Casp6
• NCBI Official Synonym Symbols: Mch2; CASP-6
• NCBI Protein Information: caspase-6
• UniProt Protein Name: Caspase-6
• Protein Family: Caspase
• UniProt Gene Name: Casp6
• UniProt Synonym Gene Names: CASP-6

NCBI Description

This gene encodes a member of the cysteine proteases that plays important roles in regulating apoptosis and neurodegeneration. The encoded protein is involved in the transmission of pain and axonal degeneration. Genetic deletion of this gene in mice results in the delay of axon pruning and protects from axon degeneration. [provided by RefSeq, Apr 2015]

Uniprot Description

CASP6: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Activation is suppressed by phosphorylation at Ser-257. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.4.22.59; Protease

Chromosomal Location of Human Ortholog: 3|3 G3

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; hydrolase activity; identical protein binding; peptidase activity; protein binding

Biological Process: acute inflammatory response to non-antigenic stimulus; apoptosis; positive regulation of apoptosis; positive regulation of neuron apoptotic process; positive regulation of retinal cell programmed cell death; proteolysis; regulation of apoptosis; response to cytokine; response to glucose stimulus; response to hydrogen peroxide; response to iron ion