Calpain, Small Subunit 1 Recombinant Protein | CAPNS1 recombinant protein

Recombinant Human Calpain, Small Subunit 1 Protein

Cat. Num. AAA556334

• Gene Names: CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4
• Purity: Greater than 95.0% as determined by: Analysis by SDS-PAGE.
• Synonyms: Calpain; Small Subunit 1; Recombinant Human Calpain; Small Subunit 1 Protein; CAPNS1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by: Analysis by SDS-PAGE.
• Form/Format: Liquid; The CAPNS1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSRTHYSNI EANESEEVRQ FRRLFAQLAG DDMEVSATEL MNILNKVVTR HPDLKTDGFG IDTCRSMVAV MDSDTTGKLG FEEFKYLWNN IKRWQAIYKQ FDTDRSGTIC SSELPGAFEA AGFHLNEHLY NMIIRRYSDE SGNMDFDNFI SCLVRLDAMF RAFKSLDKDG TGQIQVNIQE WLQLTMYS

• Species: Human
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage, it is recommended to add a carrier protein (0.15% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for CAPNS1 recombinant protein

Calpain, Small Subunit 1 (CAPNS1) belongs to the calpain small subunit family. Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases, widely distributed in mammalian cells. Calpain families are implicated in neurodegenerative processes, considering that their activation can be triggered by calcium influx and oxidative stress. Calpains function as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by the CAPNS1 gene. The CAPNS1 protein is vital for the stability and function of both calpain heterodimers, whose proteolytic activities influence numerous cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy.

CAPNS1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 208 amino acids (84-268) and having a molecular mass of 23.8kDa.

CAPNS1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

NCBI and Uniprot Product Information

• NCBI GI #: 115612
• NCBI GeneID: 826
• UniProt Accession #: P04632; Q8WTX3; Q96EW0; A8K0P1
• Molecular Weight: 268
• NCBI Official Full Name: Calpain small subunit 1
• NCBI Official Synonym Full Names: calpain, small subunit 1
• NCBI Official Symbol: CAPNS1
• NCBI Official Synonym Symbols: CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4
• NCBI Protein Information: calpain small subunit 1; CANP small subunit; calpain regulatory subunit; calpain, small polypeptide; calpain 4, small subunit (30K); calcium-dependent protease, small subunit; calcium-dependent protease small subunit 1; calcium-activated neutral proteinas
• UniProt Protein Name: Calpain small subunit 1
• Protein Family: Calpain
• UniProt Gene Name: CAPNS1
• UniProt Synonym Gene Names: CAPN4; CAPNS; CSS1; CANP small subunit; CDPS
• UniProt Entry Name: CPNS1_HUMAN

NCBI Description

This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

CAPNS1: Regulatory subunit of the calcium-regulated non- lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Protein type: Protease; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; positive regulation of cell proliferation; proteolysis