Calpain 10 Recombinant Protein | CAPN10 recombinant protein

Recombinant Calpain 10 (CAPN10)

Cat. Num. AAA2031031

• Gene Names: CAPN10; CANP10; NIDDM1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Calpain 10; Recombinant Calpain 10 (CAPN10); CAPN10 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; SDSSLFYNL STPLAQFRED ITWRRPQEIC ATPQLFPDNP WEGQVKQGLL GDCWFLCACA ALQKSQHLLD QVFPPGQPGW SDQKYQGFFT CRIWQFGHWE EVTIDDRLPC LAGRLCFSRC QREDVFWLPL LEKAYAKVHG SYEHLWAGQV ADALVDLTGS LAERWSLKDV TKASGQQDRP SGGEHRTCRQ LLHLKDRCLI SCSVLSPRAG ARELGEFHAF IISDLQELRS QTGQGILLLR IHNPWGRRCW QGLWREGGEG WNQVEPAKES ELLAQLQEGE FWVEEEEFLR EFDEVTIGYP VTEAGHLQSL HTERVLCHTR TLPGAWVTGQ SAGGCRNNSCFPCNPKFWLR LLEP
• Sequence Length: 672

• Applicable Applications for CAPN10 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 44.1kDa
• Accurate Molecular Mass (KD): 44kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Ser22~Pro374
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 37589919
• NCBI GeneID: 11132
• Molecular Weight: 15,275 Da
• NCBI Official Full Name: Calpain 10
• NCBI Official Synonym Full Names: calpain 10
• NCBI Official Symbol: CAPN10
• NCBI Official Synonym Symbols: CANP10; NIDDM1
• NCBI Protein Information: calpain-10
• UniProt Protein Name: Calpain-10
• Protein Family: Calpain
• UniProt Gene Name: CAPN10
• UniProt Synonym Gene Names: KIAA1845; CANP 10
• UniProt Entry Name: CAN10_HUMAN

NCBI Description

Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. [provided by RefSeq, Sep 2010]

Uniprot Description

CAPN10: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Defects in CAPN10 are a cause of susceptibility to diabetes mellitus non-insulin-dependent type 1 (NIDDM1). It is a multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Belongs to the peptidase C2 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.4.22.-; Mitochondrial; Protease

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: mitochondrion; cell; plasma membrane; cytosol

Molecular Function: SNARE binding; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity

Biological Process: positive regulation of glucose import; cellular response to insulin stimulus; positive regulation of intracellular transport; actin cytoskeleton reorganization; positive regulation of insulin secretion; proteolysis; cell structure disassembly during apoptosis

Disease: Diabetes Mellitus, Noninsulin-dependent, 1