Canine Transmembrane Protein 27 (TMEM27) ELISA Kit | TMEM27 elisa kit

Canine Transmembrane Protein 27 (TMEM27) ELISA Kit

Cat. Num. AAA2021070

• Gene Names: TMEM27; NX17; NX-17
• Reactivity: Canine
• Synonyms: Transmembrane Protein 27 (TMEM27); Canine Transmembrane Protein 27 (TMEM27) ELISA Kit; NX17; Collectrin; TMEM27 elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transmembrane Protein 27 (TMEM27).; No significant cross-reactivity or interference between Transmembrane Protein 27 (TMEM27) and analogues was observed.
• Sequence Length: 222

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transmembrane Protein 27 (TMEM27) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transmembrane Protein 27 (TMEM27) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TMEM27 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protein 27 (TMEM27). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transmembrane Protein 27 (TMEM27). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane Protein 27 (TMEM27), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transmembrane Protein 27 (TMEM27) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TMEM27 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119619300
• NCBI GeneID: 57393
• UniProt Accession #: Q9HBJ8; Q6UW07; B2R9M1
• Molecular Weight: 25,235 Da
• NCBI Official Full Name: transmembrane protein 27
• NCBI Official Synonym Full Names: transmembrane protein 27
• NCBI Official Symbol: TMEM27
• NCBI Official Synonym Symbols: NX17; NX-17
• NCBI Protein Information: collectrin
• UniProt Protein Name: Collectrin
• Protein Family: Collectrin
• UniProt Gene Name: TMEM27
• UniProt Entry Name: TMM27_HUMAN

NCBI Description

This gene encodes a type 1 transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. The encoded protein binds to amino acid transporters and regulates their expression on the plasma membrane. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. The extracellular domain of the encoded protein may be cleaved and shed from the plasma membrane specifically in pancreatic beta cells. [provided by RefSeq, Jun 2013]

Uniprot Description

TMEM27: Regulator of SNARE complex function. Stimulator of beta cell replication. Belongs to the TMEM27 family.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: brush border membrane; cytoplasm; integral to membrane

Molecular Function: peptidyl-dipeptidase activity; protein binding; metallopeptidase activity

Biological Process: positive regulation of calcium ion-dependent exocytosis; proteolysis; positive regulation of amino acid transport