Typical Testing Data/Standard Curve
Canine Low affinity immunoglobulin gamma Fc region receptor III-A (FCGR3A) ELISA Kit | FCGR3A elisa kit
Canine Low affinity immunoglobulin gamma Fc region receptor III-A (FCGR3A) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||FCGR3A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FCGR3A antibody and an FCGR3A-HRP conjugate. The assay sample and buffer are incubated together with FCGR3A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FCGR3A concentration since FCGR3A from samples and FCGR3A-HRP conjugate compete for the anti-FCGR3A antibody binding site. Since the number of sites is limited, as more sites are occupied by FCGR3A from the sample, fewer sites are left to bind FCGR3A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FCGR3A concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
FCGR3A: Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q23
Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane
Molecular Function: IgG binding
Biological Process: regulation of immune response; innate immune response; immune response
Disease: Immunodeficiency 20
Research Articles on FCGR3A
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Product Notes
The Canine FCGR3A fcgr3a (Catalog #AAA7234092) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7234092 ELISA Kit recognizes Canine FCGR3A. It is sometimes possible for the material contained within the vial of "Low affinity immunoglobulin gamma Fc region receptor III-A (FCGR3A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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