Rabbit anti-Human, Mouse CDIPT Polyclonal Antibody | anti-CDIPT antibody

CDIPT, ID (CDIPT, PIS, PIS1, CDP-diacylglycerol-inositol 3-phosphatidyltransferase, Phosphatidylinositol synthase) (Azide free) (HRP)

Cat. Num. AAA6284219

• Gene Names: CDIPT; PIS; PIS1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CDIPT; Polyclonal Antibody; ID (CDIPT; PIS; PIS1; CDP-diacylglycerol-inositol 3-phosphatidyltransferase; Phosphatidylinositol synthase) (Azide free) (HRP); CDP-diacylglycerol--inositol 3-phosphatidyltransferase; Phosphatidylinositol synthase; anti-CDIPT antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CDIPT. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CDIPT antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 99-125 in the central region of human CDIPT. Species sequence homology: rat
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse stomach tissue lysates (35ug/lane) usingMBS6010012. CDIPT (arrow) was detected usingMBS6010012.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted withMBS6010012, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6010012 for immunohistochemistry.)

Testing Data

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Product Categories/Family for anti-CDIPT antibody

Antibodies; Enzymes; Synthase

References

1. de Serres, F.J., et.al., Monaldi Arch Chest Dis 63 (3), 133-141 (2005). 2. Lykidis, A., et.al., J. Biol. Chem. 272 (52), 33402-33409 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 5453906
• NCBI GeneID: 10423
• NCBI Accession #: NP_006310.1
• NCBI GenBank Nucleotide #: NM_006319.3
• UniProt Accession #: O14735; Q6FGU1; Q6ZN70
• Molecular Weight: 23,539 Da
• NCBI Official Full Name: CDP-diacylglycerol--inositol 3-phosphatidyltransferase
• NCBI Official Synonym Full Names: CDP-diacylglycerol--inositol 3-phosphatidyltransferase
• NCBI Official Symbol: CDIPT
• NCBI Official Synonym Symbols: PIS; PIS1
• NCBI Protein Information: CDP-diacylglycerol--inositol 3-phosphatidyltransferase; PI synthase; PtdIns synthase; phosphatidylinositol synthase
• UniProt Protein Name: CDP-diacylglycerol--inositol 3-phosphatidyltransferase
• UniProt Gene Name: CDIPT
• UniProt Synonym Gene Names: PIS; PIS1; PI synthase
• UniProt Entry Name: CDIPT_HUMAN

NCBI Description

Phosphatidylinositol breakdown products are ubiquitous second messengers that function downstream of many G protein-coupled receptors and tyrosine kinases regulating cell growth, calcium metabolism, and protein kinase C activity. Two enzymes, CDP-diacylglycerol synthase and phosphatidylinositol synthase, are involved in the biosynthesis of phosphatidylinositol. Phosphatidylinositol synthase, a member of the CDP-alcohol phosphatidyl transferase class-I family, is an integral membrane protein found on the cytoplasmic side of the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content. The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme.

Catalytic activity: CDP-diacylglycerol + myo-inositol = CMP + phosphatidyl-1D-myo-inositol.

Cofactor: Magnesium.Manganese.

Enzyme regulation: Inhibited by PtdIns (product inhibition), phosphatidylinositol phosphate, and nucleoside di- and tri-phosphates.

Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein

By similarity. Golgi apparatus membrane; Multi-pass membrane protein

By similarity. Cell membrane; Multi-pass membrane protein

By similarity.

Tissue specificity: Widely expressed. Higher expression in adult liver and skeletal muscle, slightly lower levels seen in pancreas, kidney, lung, placenta, brain, heart, leukocyte, colon, small intestine, ovary, testis, prostate, thymus and spleen. In fetus, expressed in kidney, liver, lung and brain.

Sequence similarities: Belongs to the CDP-alcohol phosphatidyltransferase class-I family.

Biophysicochemical propertiespH dependence:Optimum pH is 9.0.Temperature dependence:Optimum temperature is 50 degrees Celsius.