Typical Testing Data/Standard Curve (for reference only)
Canine Cytochrome P450 2D6 (CYP2D6) ELISA Kit | CYP2D6 elisa kit
Canine Cytochrome P450 2D6 (CYP2D6) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CYP2D6 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CYP2D6 antibody and an CYP2D6-HRP conjugate. The assay sample and buffer are incubated together with CYP2D6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP2D6 concentration since CYP2D6 from samples and CYP2D6-HRP conjugate compete for the anti-CYP2D6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP2D6 from the sample, fewer sites are left to bind CYP2D6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP2D6 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize as many as 25% of commonly prescribed drugs. Its substrates include antidepressants, antipsychotics, analgesics and antitussives, beta adrenergic blocking agents, antiarrythmics and antiemetics. The gene is highly polymorphic in the human population; certain alleles result in the poor metabolizer phenotype, characterized by a decreased ability to metabolize the enzyme's substrates. Some individuals with the poor metabolizer phenotype have no functional protein since they carry 2 null alleles whereas in other individuals the gene is absent. This gene can vary in copy number and individuals with the ultrarapid metabolizer phenotype can have 3 or more active copies of the gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
Uniprot Description
CYP2D6: Responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It is involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. Belongs to the cytochrome P450 family.
Protein type: EC 1.14.14.1; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum
Molecular Function: iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxidoreductase activity; drug binding; oxygen binding; monooxygenase activity
Biological Process: steroid metabolic process; coumarin metabolic process; monoterpenoid metabolic process; isoquinoline alkaloid metabolic process; negative regulation of binding; drug catabolic process; xenobiotic metabolic process; exogenous drug catabolic process; arachidonic acid metabolic process; alkaloid metabolic process; alkaloid catabolic process; heterocycle metabolic process; drug metabolic process
Disease: Drug Metabolism, Poor, Cyp2d6-related
Research Articles on CYP2D6
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Product Notes
The Canine CYP2D6 cyp2d6 (Catalog #AAA7228798) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7228798 ELISA Kit recognizes Canine CYP2D6. It is sometimes possible for the material contained within the vial of "Cytochrome P450 2D6 (CYP2D6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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