Canine Cx3Cl1/Fractalkine ELISA Kit | CX3CL1 elisa kit

Canine Cx3Cl1/Fractalkine ELISA Kit

Cat. Num. AAA13787

• Gene Names: CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
• Reactivity: Canine
• Synonyms: Cx3Cl1/Fractalkine; Canine Cx3Cl1/Fractalkine ELISA Kit; CX3CL1 elisa kit

• Reactivity: Canine
• Specificity: No significant cross-reactivity or interference between Canine CX3CL1 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.5 ng/ml - 16 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated CX3CL1 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine CX3CL1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CX3CL1 and CX3CL1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CX3CL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for CX3CL1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated CX3CL1 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine CX3CL1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CX3CL1 and CX3CL1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CX3CL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 48146969
• NCBI GeneID: 6376
• UniProt Accession #: Q6I9S9; P78423
• Molecular Weight: 42,187 Da
• NCBI Official Full Name: CX3CL1
• NCBI Official Synonym Full Names: chemokine (C-X3-C motif) ligand 1
• NCBI Official Symbol: CX3CL1
• NCBI Official Synonym Symbols: NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
• NCBI Protein Information: fractalkine; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)
• UniProt Protein Name: CX3CL1 protein
• Protein Family: Fractalkine
• UniProt Gene Name: CX3CL1
• UniProt Entry Name: Q6I9S9_HUMAN

Uniprot Description

CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; cell surface; extracellular region; plasma membrane; integral to membrane

Molecular Function: protein binding; chemokine activity; receptor binding

Biological Process: neutrophil chemotaxis; positive regulation of transforming growth factor-beta1 production; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; defense response; angiogenesis involved in wound healing; chemotaxis; leukocyte adhesive activation; macrophage chemotaxis; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of inflammatory response