Canine Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit | CD40L elisa kit

Canine Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit

Cat. Num. AAA454063

• Gene Names: CD40LG; CD154; TNFSF5
• Reactivity: Canine
• Synonyms: Cluster Of Differentiation 40 Ligand (CD40L); Canine Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit; CD154; TRAP; HIGM1; IGM; IMD3; TBAM; T-BAM; TNFSF5; Gp39; TNF Superfamily Member 5; Hyper-IgM Syndrome; TNF-Related Activation Protein; T-Cell B-Cell Activating Molecule; CD40L elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of CD40L. No significant cross-reactivity or interference between CD40L and analogues was observed.
• Sequence Length: 260

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.032ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CD40L were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CD40L were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD40L elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CD40L in canine serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD40L. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CD40L in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 50950205
• NCBI GeneID: 403468
• NCBI Accession #: NP_001002981.1
• NCBI GenBank Nucleotide #: NM_001002981.1
• UniProt Accession #: O97626
• Molecular Weight: 28,688 Da
• NCBI Official Full Name: CD40 ligand
• NCBI Official Symbol: CD40LG
• NCBI Official Synonym Symbols: CD154; TNFSF5
• NCBI Protein Information: CD40 ligand
• UniProt Protein Name: CD40 ligand
• UniProt Gene Name: CD40LG
• UniProt Synonym Gene Names: CD40L; TNFSF5; CD40-L

Uniprot Description

Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching.