Chk1 Substrate Peptide Cancer Marker
Chk1 Substrate Peptide, phosphorylated (Ser) (Chktide) (CHEK1, Cell Cycle Checkpoint Kinase) (Biotin)
Purity
Highly PurifiedHighly Purified (Determined by HPLC and mass spectroscopy).
Synonyms
Chk1 Substrate Peptide; phosphorylated (Ser) (Chktide) (CHEK1; Cell Cycle Checkpoint Kinase) (Biotin); Chk1 Substrate Peptide cancer marker
Purity/Purification
Highly Purified
Highly Purified (Determined by HPLC and mass spectroscopy).
Highly Purified (Determined by HPLC and mass spectroscopy).
Form/Format
Supplied as a lyophilized powder.
Application Notes
Suitable for use in protein kinase assay.
Dilution: Protein Kinase Assay: 100ng of CHK1, active to phosphorylate the biotinylated peptide.
Dilution: Protein Kinase Assay: 100ng of CHK1, active to phosphorylate the biotinylated peptide.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Stable for 12 months at -20 degree C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Chk1 Substrate Peptide cancer marker
Synthetic peptide ([biotin-KKKVSRSGLYR(pS)PSMPENLNRPR) (linear) biotinylated at the N-terminus, phosphorylated Serine. Substrate for CHK enzymes (C4200-10A).
Chk1, one of the downstream protein kinases of ATM/ATR, plays an important role in DNA damage checkpoint, embryonic development and tumor suppression. The activation of Chk1 in response to replication blocks and certain forms of genotoxic stress involves phosphorylation of serines 317 and 345. Activated Chk1 can inactivate cdc25c via phosphorylation at serine 216, blocking the activation of cdc2 and transition into M-phase. Chk1 can also phosphorylate p53 at serine 20 in vitro.
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2-DNA damage checkpoint by phosphorylating CDC25C in response to DNA damage. CDC25A, CDC25B and CDC25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating CDC2 p34 on regulatory tyrosine residues. Phosphorylation of CDC25C by Chk1 and 2 inhibits its phosphatase activity, thereby blocking mitosis. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.
Chk1, one of the downstream protein kinases of ATM/ATR, plays an important role in DNA damage checkpoint, embryonic development and tumor suppression. The activation of Chk1 in response to replication blocks and certain forms of genotoxic stress involves phosphorylation of serines 317 and 345. Activated Chk1 can inactivate cdc25c via phosphorylation at serine 216, blocking the activation of cdc2 and transition into M-phase. Chk1 can also phosphorylate p53 at serine 20 in vitro.
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2-DNA damage checkpoint by phosphorylating CDC25C in response to DNA damage. CDC25A, CDC25B and CDC25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating CDC2 p34 on regulatory tyrosine residues. Phosphorylation of CDC25C by Chk1 and 2 inhibits its phosphatase activity, thereby blocking mitosis. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.
Product Categories/Family for Chk1 Substrate Peptide cancer marker
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Product Notes
The Chk1 Substrate Peptide (Catalog #AAA651679) is a Cancer Marker and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in protein kinase assay. Dilution: Protein Kinase Assay: 100ng of CHK1, active to phosphorylate the biotinylated peptide. Researchers should empirically determine the suitability of the Chk1 Substrate Peptide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chk1 Substrate Peptide, Cancer Marker" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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