Bovine Interferon Gamma (IFN-gamma) ELISA Kit | IFN-gamma elisa kit

Bovine Interferon Gamma (IFN-gamma) ELISA Kit

Cat. Num. AAA2609318

• Gene Names: IFNG; IFG; IFI
• Reactivity: Bovine
• Synonyms: Interferon Gamma (IFN-gamma); Bovine Interferon Gamma (IFN-gamma) ELISA Kit; IFN-gamma elisa kit

• Reactivity: Bovine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 166

• Samples: Serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 1000 pg/ml-15.6 pg/ml
• Sensitivity: Up to 5 pg/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for IFN-gamma elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Bovine IFN-gamma monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 56786138
• NCBI GeneID: 3458
• NCBI Accession #: NP_000610.2
• NCBI GenBank Nucleotide #: NM_000619.2
• Molecular Weight: 19,348 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon gamma
• NCBI Official Symbol: IFNG
• NCBI Official Synonym Symbols: IFG; IFI
• NCBI Protein Information: interferon gamma
• UniProt Protein Name: Interferon gamma
• UniProt Gene Name: IFNG
• UniProt Synonym Gene Names: IFN-gamma
• UniProt Entry Name: IFNG_HUMAN

NCBI Description

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

Uniprot Description

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Cytokine; Membrane protein, integral; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: extracellular region; extracellular space

Molecular Function: cytokine activity

Biological Process: adaptive immune response; apoptosis; cell cycle arrest; cell motility; cell surface receptor linked signal transduction; humoral immune response; negative regulation of epithelial cell differentiation; negative regulation of interleukin-17 production; negative regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of autophagy; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation during immune response; positive regulation of cell proliferation; positive regulation of interleukin-12 production; positive regulation of interleukin-23 production; positive regulation of killing of cells of another organism; positive regulation of membrane protein ectodomain proteolysis; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoclast differentiation; positive regulation of peptidyl-serine phosphorylation of STAT protein; positive regulation of tyrosine phosphorylation of Stat1 protein; protein import into nucleus, translocation; regulation of insulin secretion; response to virus

Disease: Aplastic Anemia; Hepatitis C Virus, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2