Gene Sequencing (extract)
Calcitonin Gene Related Peptide (CGRP) Recombinant Protein | CGRP recombinant protein
Recombinant Calcitonin Gene Related Peptide (CGRP)
Gene Names
CALCB; CALC2; CGRP2; CGRP-II
Reactivity
Homo sapiens (Human)
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Calcitonin Gene Related Peptide (CGRP); Recombinant Calcitonin Gene Related Peptide (CGRP); CGRP recombinant protein
Host
E.coli
Reactivity
Homo sapiens (Human)
Purity/Purification
> 95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Sequence
MGFRKFSPFL ALSILVLYQA GSLQAAPFRS ALESSPDPAT LSKEDARLLL AALVQDYVQM KASELKQEQE TQGSSSAAQK RACNTATCVT HRLAGLLSRS GGMVKSNFVP TNVGSKAFGR RRRDLQA
Applicable Applications for CGRP recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Met1~Ala127
Tags
N-terminal His Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
9.9
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Gene Sequencing (extract)
Gene Sequencing (extract)
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 15.0kDa
Accurate Molecular Mass: 19kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 19kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
calcitonin gene-related peptide 2
NCBI Official Synonym Full Names
calcitonin-related polypeptide beta
NCBI Official Symbol
CALCB
NCBI Official Synonym Symbols
CALC2; CGRP2; CGRP-II
NCBI Protein Information
calcitonin gene-related peptide 2; beta-CGRP; calcitonin 2; beta-type CGRP; calcitonin gene-related peptide II
UniProt Protein Name
Calcitonin gene-related peptide 2
UniProt Gene Name
CALCB
UniProt Synonym Gene Names
CALC2; Beta-CGRP; CGRP-II
UniProt Entry Name
CALCB_HUMAN
Uniprot Description
CALCB: CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. Belongs to the calcitonin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11p15.2-p15.1
Cellular Component: extracellular region
Molecular Function: neuropeptide hormone activity
Biological Process: cellular calcium ion homeostasis; signal transduction
Research Articles on CGRP
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Product Notes
The CGRP calcb (Catalog #AAA2009016) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Calcitonin Gene Related Peptide (CGRP) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Calcitonin Gene Related Peptide (CGRP) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the CGRP calcb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGFRKFSPFL ALSILVLYQA GSLQAAPFRS ALESSPDPAT LSKEDARLLL AALVQDYVQM KASELKQEQE TQGSSSAAQK RACNTATCVT HRLAGLLSRS GGMVKSNFVP TNVGSKAFGR RRRDLQA. It is sometimes possible for the material contained within the vial of "Calcitonin Gene Related Peptide (CGRP), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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