Human calcitonin-related polypeptide alpha ELISA Kit | CALCA elisa kit

Human Calcitonin, CT ELISA Kit

Cat. Num. AAA702461

• Gene Names: CALCA; CT; KC; CGRP; CALC1; CGRP1; CGRP-I
• Reactivity: Human
• Synonyms: calcitonin-related polypeptide alpha; Human Calcitonin; CT ELISA Kit; CALC1; CGRP; CGRP-I; CGRP1; CT; KC; MGC126648; calcitonin 1; calcitonin gene-related peptide 1; calcitonin/calcitonin-related polypeptide; alpha; katacalcin; CALCA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CALCA. No significant cross-reactivity or interference between Human CALCA and analogues was observed.
• Sequence Length: 141

• Samples: Serum, plasma and tissue homogenates
• Detection Range: 4.7 pg/ml-300 pg/ml
• Sensitivity: 1.175 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CALCA elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcitonin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Calcitonin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Calcitonin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Calcitonin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 76880484
• NCBI GeneID: 796
• NCBI Accession #: NP_001029124.1
• NCBI GenBank Nucleotide #: NM_001033952.2
• UniProt Accession #: P01258; Q13935; Q13937; Q52LX7; B7ZL39; P06881
• Molecular Weight: 15,466 Da
• NCBI Official Full Name: calcitonin isoform CT preproprotein
• NCBI Official Synonym Full Names: calcitonin-related polypeptide alpha
• NCBI Official Symbol: CALCA
• NCBI Official Synonym Symbols: CT; KC; CGRP; CALC1; CGRP1; CGRP-I
• NCBI Protein Information: calcitonin; calcitonin gene-related peptide 1; katacalcin; calcitonin 1; alpha-type CGRP; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha
• UniProt Protein Name: Calcitonin
• Protein Family: Calcitonin
• UniProt Gene Name: CALCA
• UniProt Synonym Gene Names: CALC1; CCP
• UniProt Entry Name: CALC_HUMAN

NCBI Description

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]

Uniprot Description

Function: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.