CACYBP recombinant protein

CACYBP, 1-185aa, Human, His tag, E Coli

Cat. Num. AAA203503

• Gene Names: CACYBP; SIP; GIG5; PNAS-107; S100A6BP
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: CACYBP; 1-185aa; Human; His tag; E Coli; Clcyclin-binding protein; GIG5; PNAS-107; RP1-102G20.6; S100A6BP; SIP; Clcyclin-binding protein CACYBP; Calcyclin binding protein; GIG 5; Growth inhibiting gene 5; Growth inhibiting gene 5 protein; hCacyBP; MGC87971; PNAS 107; PNAS107; RP1 102G20.6; S100A6 binding protein.; CACYBP recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MQQKSQKKAE LLDNEKPAAV VAPITTGYTV KISNYGWDQS DKFVKIYITL TGVHQVPTEN VQVHFTERSF DLLVKNLNGK SYSMIVNNLL KPISVEGSSK KVKTDTVLIL CRKKVENTRW DYLTQVEKEC KEKEKPSYDT ETDPSEGLMN VLKKIYEDGD DDMKRTINKA WVESREKQAK GDTEF
• Sequence Length: 185

• Applicable Applications for CACYBP recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CACYBP recombinant protein

CACYBP is primarily a nuclear protein that contains one CS domain and one SGS domain. It is believed to be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It most likely serves as a molecular bridge in ubiquitin E3 complexes. It also participates in the ubiquitin-mediated degradation of beta-catenin. CACYBP is thought to be a potential inhibitor of cell growth and invasion in the gastric cancer cell through its effects on beta-catenin protein expression and transcriptional activation of TCF/LEF. Recombinant human CACYBP protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CACYBP recombinant protein

Ubiquitination

References

Matsuzawa SI., et al. (2001) Mol Cell. 7 (5):915-26. ; Filipek A., et al. (2002) J Biol Chem. 277 (23):21103-9.;

NCBI and Uniprot Product Information

• NCBI GI #: 60218913
• NCBI GeneID: 27101
• NCBI Accession #: NP_001007215
• NCBI GenBank Nucleotide #: NM_001007214.1
• UniProt Accession #: Q9HB71; O60666; Q5R370; Q5R371; B2ZWH2; B3KSF1
• Molecular Weight: 23.4 kDa (205aa), confirmed by MALDI-TOF
• NCBI Official Full Name: calcyclin-binding protein isoform 2
• NCBI Official Synonym Full Names: calcyclin binding protein
• NCBI Official Symbol: CACYBP
• NCBI Official Synonym Symbols: SIP; GIG5; PNAS-107; S100A6BP
• NCBI Protein Information: calcyclin-binding protein
• UniProt Protein Name: Calcyclin-binding protein
• Protein Family: Calcyclin-binding protein
• UniProt Gene Name: CACYBP
• UniProt Synonym Gene Names: S100A6BP; SIP; CacyBP; hCacyBP

NCBI Description

The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).