Human Carbonic anhydrase 4 ELISA Kit | CA4 elisa kit

Human Carbonic anhydrase 4 ELISA Kit

Cat. Num. AAA762737

• Gene Names: Car4; Ca4
• Reactivity: Human
• Synonyms: Carbonic anhydrase 4; Human Carbonic anhydrase 4 ELISA Kit; CA4 (Carbonic Anhydrase IV)/CAIV/CA-IV/Car4/RP17/Carbonate dehydratase IV; CA4 elisa kit

• Reactivity: Human
• Sequence Length: 309

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CA4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CA4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CA4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CA4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CA4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 9506449
• NCBI GeneID: 29242
• NCBI Accession #: NP_062047.1
• NCBI GenBank Nucleotide #: NM_019174.3
• Molecular Weight: 35,076 Da
• NCBI Official Full Name: carbonic anhydrase 4
• NCBI Official Synonym Full Names: carbonic anhydrase 4
• NCBI Official Symbol: Car4
• NCBI Official Synonym Symbols: Ca4
• NCBI Protein Information: carbonic anhydrase 4
• UniProt Protein Name: Carbonic anhydrase 4
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: Ca4
• UniProt Synonym Gene Names: CA-IV
• UniProt Entry Name: CAH4_RAT

NCBI Description

may be involved in carbon dioxide exchange and pH reguation [RGD, Feb 2006]

Uniprot Description

CA4: Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. Defects in CA4 are the cause of retinitis pigmentosa type 17 (RP17). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP17 inheritance is autosomal dominant. Defective acid overload removal from retina and retinal epithelium, due to mutant CA4, is responsible for photoreceptor degeneration, indicating that impaired pH homeostasis is the most likely cause underlying the RP17 phenotype. Belongs to the alpha-carbonic anhydrase family.

Protein type: Vesicle; Lyase; Membrane protein, anchored; Endoplasmic reticulum; Cell surface; Energy Metabolism - nitrogen; EC 4.2.1.1; Membrane protein, GPI anchor

Cellular Component: anchored to external side of plasma membrane; anchored to plasma membrane; apical plasma membrane; basolateral plasma membrane; brush border membrane; cell surface; ER-Golgi intermediate compartment; Golgi apparatus; membrane; perinuclear region of cytoplasm; plasma membrane; rough endoplasmic reticulum; sarcolemma; sarcoplasmic reticulum; secretory granule membrane; trans-Golgi network

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process; organ development; response to drug; response to steroid hormone stimulus